小鼠体内75硒标记布氏锥虫的免疫清除。I. 放射性标记技术的若干方面。
Immunological clearance of 75Se-labelled Trypanosoma brucei in mice. I. Aspects of the radiolabelling technique.
作者信息
Holmes P H, MacAskill J A, Whitelaw D D, Jennings F W, Urquhart G M
出版信息
Immunology. 1979 Mar;36(3):415-20.
Abstract
A reliable and simple technique for the in vivo labelling of Trypanosoma brucei with [75Se]-methionine was developed. Between 97 and 99% of the radioactivity was protein bound in the trypanosomes and spontaneous elution in vitro was less than 10% over 4 h. The fate of the labelled trypanosomes after i.v. injection into normal and immune mice was studied. Whilst the vast majority of parasites remained in the circulation of normal animals they rapidly disappeared from the blood of immune animals. In the latter the liver was found to be the principal site of phagocytosis removing over 50% of the radiolabelled parasites within 15 min of injection.
摘要
开发了一种用[75Se]-蛋氨酸对布氏锥虫进行体内标记的可靠且简单的技术。锥虫中97%至99%的放射性与蛋白质结合，体外4小时内的自发洗脱率低于10%。研究了静脉注射到正常和免疫小鼠体内后标记锥虫的命运。虽然绝大多数寄生虫仍留在正常动物的循环中，但它们在免疫动物的血液中迅速消失。在后者中，发现肝脏是吞噬作用的主要部位，在注射后15分钟内清除了超过50%的放射性标记寄生虫。
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