通过测量生长中酶分子的浓度研究激素诱导酪氨酸转氨酶的机制。
Mechanism of hormonal induction of tyrosine aminotransferase studied by measurement of the concentration of growing enzyme molecules.
作者信息
Scott W A, Shields R, Tomkins G M
出版信息
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2937-41. doi: 10.1073/pnas.69.10.2937.
Abstract
Proteins labeled in cultured hepatoma cells grown in the presence of radioactive amino acids and pactamycin consist primarily of growing polypeptide chains initiated before addition of the antibiotic. Nascent tyrosine aminotransferase (EC 2.6.1.5) molecules were labeled in this way, and their radioactivity after completion was measured immunologically. Cells exposed to the synthetic adrenal steroid, dexamethasone, contain 10- to 18- times as much nascent enzyme as uninduced cells. These results indicate that induction control is not at the level of polypeptide-chain elongation or release, but probably operates on either the amount of messenger RNA or the rate of specific polypeptide-chain initiation.
摘要
在含有放射性氨基酸和放线菌酮的条件下培养的肝癌细胞中标记的蛋白质，主要由在添加抗生素之前起始的正在生长的多肽链组成。新生的酪氨酸转氨酶（EC 2.6.1.5）分子就是以这种方式被标记的，并且在合成完成后通过免疫学方法测量其放射性。暴露于合成肾上腺皮质类固醇地塞米松的细胞，其新生酶的含量是未诱导细胞的10至18倍。这些结果表明，诱导控制并非在多肽链延伸或释放的水平上起作用，而是可能作用于信使RNA的量或特定多肽链起始的速率。
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Mechanism of hormonal induction of tyrosine aminotransferase studied by measurement of the concentration of growing enzyme molecules.通过测量生长中酶分子的浓度研究激素诱导酪氨酸转氨酶的机制。
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本文引用的文献
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Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA.高等生物中特定基因表达的调控。哺乳动物基因的表达可能受作用于信使核糖核酸翻译的阻遏物调控。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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