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The use of exonuclease III for preparing single stranded DNA for use as a template in the chain terminator sequencing method.

作者信息

Smith A J

出版信息

Nucleic Acids Res. 1979 Mar;6(3):831-48. doi: 10.1093/nar/6.3.831.

DOI:10.1093/nar/6.3.831
PMID:440973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327737/
Abstract

Exonuclease III, which degrades DNA 3' leads to 5' and is specific for duplex DNA, can be used to prepare single stranded DNA from linear duplexes. This is shown to be suitable as a template for use with the chain terminator DNA sequencing method of Sanger et al. [1]. Strategies are discussed for preparing single stranded DNA templates by this method and, in particular, its application to the sequence analysis of DNA cloned in plasmid vectors.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/a7a2c24689d8/nar00444-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/9c9e47f92d57/nar00444-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/d257c027aa1b/nar00444-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/4f10c60ac19c/nar00444-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/3eb3fa5dfca2/nar00444-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/a7a2c24689d8/nar00444-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/9c9e47f92d57/nar00444-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/d257c027aa1b/nar00444-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/4f10c60ac19c/nar00444-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/3eb3fa5dfca2/nar00444-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2ad/327737/a7a2c24689d8/nar00444-0038-a.jpg

相似文献

1
The use of exonuclease III for preparing single stranded DNA for use as a template in the chain terminator sequencing method.
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2
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本文引用的文献

1
A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.来自大肠杆菌的一种脱氧核糖核酸磷酸酶-核酸外切酶。II. 核酸外切酶活性的特性
J Biol Chem. 1964 Jan;239:251-8.
2
Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid.脱氧核糖核酸单链断裂处核苷酸的酶促去除与替换
J Biol Chem. 1971 Apr 25;246(8):2680-91.
3
Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA.DNA核苷酸序列的直接测定：噬菌体phiX172 DNA片段的结构
T5 外切核酸酶对乙型肝炎病毒复制中间体的水解作用允许通过 PCR 可靠地定量和快速检测共价闭合环状 DNA 的药物疗效。
J Virol. 2018 Nov 12;92(23). doi: 10.1128/JVI.01117-18. Print 2018 Dec 1.
4
On-Flow Synthesis of Co-Polymerizable Oligo-Microspheres and Application in ssDNA Amplification.可共聚寡聚微球的流动合成及其在单链DNA扩增中的应用。
PLoS One. 2016 Jul 22;11(7):e0159777. doi: 10.1371/journal.pone.0159777. eCollection 2016.
5
Methods for the preparation of large quantities of complex single-stranded oligonucleotide libraries.制备大量复杂单链寡核苷酸文库的方法。
PLoS One. 2014 Apr 14;9(4):e94752. doi: 10.1371/journal.pone.0094752. eCollection 2014.
6
Nucleoside alpha-thiotriphosphates, polymerases and the exonuclease III analysis of oligonucleotides containing phosphorothioate linkages.核苷α-硫代三磷酸、聚合酶以及含硫代磷酸酯键的寡核苷酸的核酸外切酶III分析
Nucleic Acids Res. 2007;35(9):3118-27. doi: 10.1093/nar/gkm168. Epub 2007 Apr 22.
7
DNA microarrays with stem-loop DNA probes: preparation and applications.带有茎环DNA探针的DNA微阵列：制备与应用
Nucleic Acids Res. 2001 Oct 1;29(19):E92. doi: 10.1093/nar/29.19.e92.
8
Neighboring plasmid sequences can affect Mini-Mu DNA transposition in the absence of expression of the bacteriophage Mu semi-essential early region.在噬菌体Mu半必需早期区域不表达的情况下，相邻的质粒序列可影响Mini-Mu DNA转座。
Arch Microbiol. 1994;161(5):418-24. doi: 10.1007/BF00288953.
9
Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyltransferase.部分编码人次黄嘌呤磷酸核糖基转移酶的基因组克隆的分离
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5038-41. doi: 10.1073/pnas.79.16.5038.
10
Variation in the polyadenylylation site of bovine prolactin mRNA.牛催乳素mRNA聚腺苷酸化位点的变异
Proc Natl Acad Sci U S A. 1982 Jan;79(2):223-7. doi: 10.1073/pnas.79.2.223.
J Mol Biol. 1974 Aug 15;87(3):377-407. doi: 10.1016/0022-2836(74)90093-x.
4
Nucleotide sequence of bacteriophage G4 DNA.噬菌体G4 DNA的核苷酸序列。
Nature. 1978 Nov 16;276(5685):236-47. doi: 10.1038/276236a0.
5
The use of thin acrylamide gels for DNA sequencing.用于DNA测序的薄丙烯酰胺凝胶的使用。
FEBS Lett. 1978 Mar 1;87(1):107-10. doi: 10.1016/0014-5793(78)80145-8.
6
Is there a correction mechanism in the 5S multigene system?
Nature. 1978 Oct 12;275(5680):556-8. doi: 10.1038/275556a0.
7
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer.非洲爪蟾卵母细胞5S DNA的核苷酸序列。I.富含AT的间隔区。
Cell. 1978 Apr;13(4):701-16. doi: 10.1016/0092-8674(78)90220-9.
8
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9
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Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.