Halliday W J, Walters B A
Clin Exp Immunol. 1974 Feb;16(2):203-12.
Guinea-pigs made tolerant to dinitrochlorobenzene (by prior intracardiac injection of dinitrobenzene sulphonate) failed to give positive skin reactions after contact sensitization, but nevertheless had peritoneal cells which reacted with the hapten in macrophage migration inhibition (MMI) tests. This reactivity was blocked by the addition of serum from tolerant animals but not by serum from hypersensitive animals. Cells from hypersensitive guinea-pigs were anomalous, in that their reaction with hapten in MMI was not blocked by tolerant serum. Hypersensitive serum, though not active by itself in MMI, was able to prevent blocking by tolerant serum when the two sera were mixed. This was interpreted as an `unblocking' phenomenon and suggested that hypersensitive cells were insusceptible to blocking because they themselves produced an unblocking substance (antibody?), although preliminary efforts to demonstrate this directly were not successful. Hypersensitive serum had an analogous activity , since when passively transferred to otherwise tolerant animals it enabled them to produce typical skin reactions; that is, it broke tolerance. Tolerance or non-reactivity in the situation investigated thus appears to be an enhancement-like process, characterized by the presence of reactive lymphoid cells and a blocking factor (antigen–antibody complex?) detectable in the serum of the tolerant animals.
经预先心内注射二硝基苯磺酸盐而对二硝基氯苯产生耐受的豚鼠，在接触致敏后未能出现阳性皮肤反应，但在巨噬细胞游走抑制（MMI）试验中，其腹腔细胞却能与该半抗原发生反应。这种反应性可被耐受动物的血清所阻断，但不能被超敏动物的血清阻断。超敏豚鼠的细胞表现异常，因为它们在MMI试验中与半抗原的反应不能被耐受血清阻断。超敏血清虽然本身在MMI试验中无活性，但当与耐受血清混合时，却能阻止耐受血清的阻断作用。这被解释为一种“解除阻断”现象，表明超敏细胞不易被阻断，是因为它们自身产生了解除阻断物质（抗体？），尽管直接证明这一点的初步尝试未获成功。超敏血清具有类似活性，因为当被动转移到原本耐受的动物体内时，能使它们产生典型的皮肤反应；也就是说，它打破了耐受。因此，在所研究的这种情况下，耐受或无反应性似乎是一个类似增强的过程，其特征是存在反应性淋巴细胞以及在耐受动物血清中可检测到的一种阻断因子（抗原 - 抗体复合物？）。
