Dekaris D, Silobrcić V, Mazuran R, Kadrnka-Lovrencić M
Clin Exp Immunol. 1974 Feb;16(2):311-20.
An expression of delayed-type hypersensitivity to tuberculin was tested in Mantoux positive and Mantoux negative persons. Their lymphocytes and monocytes were isolated from venous blood and incubated in vaseline chambers with or without tuberculin. In the presence of tuberculin, a substantially lower percentage of monocytes from Mantoux positive persons spread, than monocytes from Mantoux negative persons. This antigen-induced inhibition of monocyte spreading seems to be a reliable measure of tuberculin hypersensitivity, since it occurs only in Mantoux positive persons and correlates well with the intensity of the tuberculin skin reaction. Reproducibility of the test and the speed with which it is performed could constitute a basis for the use of monocyte spreading inhibition in clinical studies of cell-mediated immune reactions.
在结核菌素试验阳性和阴性的人群中检测了对结核菌素的迟发型超敏反应。从静脉血中分离出他们的淋巴细胞和单核细胞，并在凡士林小室中与或不与结核菌素一起孵育。在有结核菌素存在的情况下，结核菌素试验阳性者的单核细胞发生铺展的百分比明显低于结核菌素试验阴性者的单核细胞。这种抗原诱导的单核细胞铺展抑制似乎是结核菌素超敏反应的可靠指标，因为它仅发生在结核菌素试验阳性者中，并且与结核菌素皮肤反应的强度密切相关。该试验的可重复性及其操作速度可为在细胞介导免疫反应的临床研究中使用单核细胞铺展抑制提供依据。
