抗体合成控制的研究。VI. 抗原剂量和免疫后时间对小鼠抗体亲和力和异质性的影响。
Studies on the control of antibody synthesis. VI. Effect of antigen dose and time after immunization on antibody affinity and heterogeneity in the mouse.
作者信息
Kim Y T, Siskind G W
出版信息
Clin Exp Immunol. 1974 Jun;17(2):329-38.
The effect of antigen dose and time after immunization on the affinity of serum antihapten antibody was studied in the mouse by the Farr technique and by equilibrium dialysis. A progressive increase in affinity was seen with time after immunization at all antigen dose levels. The rate of increase in affinity was faster with lower doses of antigen. However, the increase in affinity continued for a longer time in animals immunization with larger doses of antigen. Consequently, at 3 months after immunization animals injected with a larger dose of antigen had higher average affinity antibody than did animals immunized with low doses of antigen. Low affinity antibody was produced in significant amounts at all immunizing dose levels and was present throughout the course of the immune response. Certain technical problems in interpretation of equilibrium dialysis data are discussed.
采用Farr技术和平衡透析法，在小鼠中研究了抗原剂量和免疫后时间对血清抗半抗原抗体亲和力的影响。在所有抗原剂量水平下，免疫后随着时间的推移，亲和力呈渐进性增加。较低剂量的抗原，亲和力增加的速率更快。然而，用较大剂量抗原免疫的动物，亲和力增加持续的时间更长。因此，免疫后3个月，注射较大剂量抗原的动物比用低剂量抗原免疫的动物具有更高的平均亲和力抗体。在所有免疫剂量水平下，均产生了大量低亲和力抗体，且在免疫反应过程中一直存在。文中讨论了平衡透析数据解释中的某些技术问题。
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