Fibach E, Hayashi M, Sachs L
Proc Natl Acad Sci U S A. 1973 Feb;70(2):343-6. doi: 10.1073/pnas.70.2.343.
Cells from a myeloid leukemic line in culture can be induced by the differentiation-inducing protein MGI to form colonies with normal differentiation to mature macrophages and granulocytes. This line consisted of clones that can be induced to undergo normal cell differentiation (D(+) clones) and clones (D(-) clones) that were not inducible. D(+) clones were able to undergo differentiation to both macrophages and granulocytes. Normal differentiation was induced even in clones that were no longer diploid. D(+) clones can segregate some D(-) progeny, and D(-) clones can segregate some D(+) progeny. This, therefore, provides a system for studies on the genetic and chemical control of cell differentiation in leukemic cells.
培养中的髓系白血病细胞系的细胞可被分化诱导蛋白MGI诱导形成具有向成熟巨噬细胞和粒细胞正常分化能力的集落。该细胞系由可被诱导进行正常细胞分化的克隆（D(+)克隆）和不可诱导的克隆（D(-)克隆）组成。D(+)克隆能够向巨噬细胞和粒细胞分化。即使在不再是二倍体的克隆中也能诱导正常分化。D(+)克隆可分离出一些D(-)后代，而D(-)克隆可分离出一些D(+)后代。因此，这为研究白血病细胞中细胞分化的遗传和化学控制提供了一个系统。
