Frazier W A, Boyd L F, Bradshaw R A
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2931-5. doi: 10.1073/pnas.70.10.2931.
Nerve growth factor was insolubilized by covalent attachment to Sepharose beads. Nerve growth factor-Sepharose was biologically active in both the neurite outgrowth assay for nerve growth factor and in preserving responsive neurons in vitro. Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. These observations extend the previously noted correlations on the structure and function of nerve growth factor and insulin to include the primary action of these two proteins. Thus nerve growth factor, like insulin, appears to express its biological activity by first binding to a receptor on the surface membrane of responsive cells.
通过与琼脂糖珠共价连接使神经生长因子不溶解。神经生长因子-琼脂糖在神经生长因子的神经突生长测定以及体外保存反应性神经元方面均具有生物活性。对生物测定进行修改以检测神经生长因子的溶解活性，并对用神经生长因子-琼脂糖处理的神经节进行组织学检查，结果显示，在6M盐酸胍中反应制备的神经生长因子-琼脂糖释放的溶解神经生长因子活性可忽略不计。这些观察结果扩展了先前关于神经生长因子和胰岛素的结构与功能的相关性，将这两种蛋白质的主要作用包括在内。因此，神经生长因子与胰岛素一样，似乎首先通过与反应性细胞表面膜上的受体结合来表达其生物活性。
