关于细菌视紫红质中氢-氘交换的机制
On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin.
作者信息
Doukas A G, Pande A, Suzuki T, Callender R H, Honig B, Ottolenghi M
出版信息
Biophys J. 1981 Feb;33(2):275-9. doi: 10.1016/S0006-3495(81)84889-8.
Abstract
Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.
摘要
在细菌视紫红质中进行的连续流动共振拉曼实验表明，席夫碱上的氘核与质子的交换发生在短于3毫秒的时间内。基于碱催化去质子化随后席夫碱再质子化的交换机制被排除。提出了一种机制，其中水分子在协同交换机制中直接与席夫碱氘核相互作用。似乎在黑暗中，结合位点对中性水分子的可及性比对带电质子的可及性更高。
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本文引用的文献
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Hydrogen exchange.氢交换
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Test reactions for a stopped-flow apparatus. Reduction of 2,6-dichlorophenolindophenol and potassium ferricyanide by L-ascorbic acid.停流装置的测试反应。L-抗坏血酸对2,6-二氯酚靛酚和铁氰化钾的还原反应。
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Photoisomerization, energy storage, and charge separation: a model for light energy transduction in visual pigments and bacteriorhodopsin.光异构化、能量存储与电荷分离：视觉色素和细菌视紫红质中光能转导的一个模型
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Bacteriorhodopsin and the purple membrane of halobacteria.细菌视紫红质与嗜盐菌的紫膜
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