Doukas A G, Pande A, Suzuki T, Callender R H, Honig B, Ottolenghi M
Biophys J. 1981 Feb;33(2):275-9. doi: 10.1016/S0006-3495(81)84889-8.
Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.
在细菌视紫红质中进行的连续流动共振拉曼实验表明，席夫碱上的氘核与质子的交换发生在短于3毫秒的时间内。基于碱催化去质子化随后席夫碱再质子化的交换机制被排除。提出了一种机制，其中水分子在协同交换机制中直接与席夫碱氘核相互作用。似乎在黑暗中，结合位点对中性水分子的可及性比对带电质子的可及性更高。
