Dauphinee M J, Talal N
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3769-72. doi: 10.1073/pnas.70.12.3769.
Thymocytes from NZB and DBA/2 mice were injected into lethally irradiated C57 B1/6 recipients. DNA synthesis in spleen and lymph node was measured as the incorporation of radioactive 5-iodo-2-deoxyuridine. The generation of cytotoxic effector cells was also studied in an in vitro cytotoxicity assay, with EL-4 lymphoma target cells. The kinetics of DNA synthesis were similar for 2- and 8-week-old DBA/2 donors and for 2-week-old NZB donors with a peak on day 4. Thymocytes from 8-week-old and 9-month-old NZB donors showed a delayed onset of DNA synthesis which was still increasing on day 6. When NZB donor thymocytes from 2- and 8-week-old mice were mixed together before injections, as few as 25% of 8-week-old cells gave a DNA synthetic response characteristic of this aged population. These results suggest an abnormal thymocyte development in NZB mice which may relate to the subsequent emergence of autoimmune disease.
将来自新西兰黑鼠（NZB）和DBA/2小鼠的胸腺细胞注射到经致死剂量照射的C57 B1/6受体小鼠体内。通过放射性5-碘-2-脱氧尿苷的掺入来测量脾脏和淋巴结中的DNA合成。还在体外细胞毒性试验中，以EL-4淋巴瘤靶细胞研究了细胞毒性效应细胞的产生。2周龄和8周龄的DBA/2供体以及2周龄的NZB供体的DNA合成动力学相似，在第4天达到峰值。来自8周龄和9月龄NZB供体的胸腺细胞显示DNA合成起始延迟，在第6天仍在增加。当将2周龄和8周龄NZB供体小鼠的胸腺细胞在注射前混合在一起时，低至25%的8周龄细胞产生了该年龄群体特有的DNA合成反应。这些结果表明NZB小鼠胸腺细胞发育异常，这可能与自身免疫性疾病随后的出现有关。
