Hedfors E, Holm G, Pettersson D
Clin Exp Immunol. 1974 Jun;17(2):219-26.
Highly purified blood lymphocytes from sarcoidosis patients were characterized by rosette technique for the presence of receptors for complement and by indirect immunofluorescence for the presence of membrane-bound immunoglobulin (B cells). T cells were estimated by spontaneous rosette formation with sheep red blood cells. The proportion of B cells was significantly higher and the proportion of T cells significantly lower than in the control group. Marked lymphocytopenia was found in the patient group. As a consequence the total number of circulating B cells was normal whereas the total number of circulating T cells was significantly depressed. Large atypical mononuclear cells were found in most patients. One-third of these cells had receptors for human complement.
通过玫瑰花结技术检测结节病患者高度纯化的血液淋巴细胞是否存在补体受体，并通过间接免疫荧光检测是否存在膜结合免疫球蛋白（B细胞）。通过与绵羊红细胞自发形成玫瑰花结来估计T细胞。与对照组相比，B细胞比例显著更高，T细胞比例显著更低。在患者组中发现明显的淋巴细胞减少。因此，循环B细胞总数正常，而循环T细胞总数显著降低。大多数患者中发现了大型非典型单核细胞。其中三分之一的细胞具有人补体受体。
