Thompson J S, Parmely M J, Flink R J, Canady M S, Severson C D
J Exp Med. 1972 Mar 1;135(3):596-609. doi: 10.1084/jem.135.3.596.
The influence of varying degrees of incompatibility for HL-A antigens on one-way mixed lymphocyte cultures (MLC) has been investigated. Reactions have been compared to a simple expression of HL-A antigens, allele compatibility, and a proposal considering the potential influence of antigen matching in relationship to allele compatibility. As expected, HL-A compatibility was associated with nonstimulated cultures, but significant correlation was not observed when incompatibility was expressed in terms of HL-A antigen or allele mismatching. When the relationship of both was considered, however, a second distinctive group was demonstrated that shared one allele plus one antigen of the second allele. Within this group no stimulation, even with augmented culture conditions, was observed in some families. Employing these same criteria, there was no significant difference in the MLC response in those groups that were incompatible for both alleles regardless of the number of matched antigens or the group that shared an allele but differed by both antigens of the second allele. These results support the concept of an intimate relationship between the loci coding for HL-A antigens and mixed culture reactions. They suggest that HL-A haplotype incompatibility acting as a unit is the primary stimulus of the MLC response, and that the immunogenicity of the haplotype also relates to whether or not one antigen is common to the stimulating and responding cell.
研究了HL - A抗原不同程度的不相容性对单向混合淋巴细胞培养（MLC）的影响。已将反应与HL - A抗原的简单表达、等位基因相容性以及考虑抗原匹配相对于等位基因相容性的潜在影响的一项提议进行了比较。正如预期的那样，HL - A相容性与未受刺激的培养相关，但当以HL - A抗原或等位基因错配来表示不相容性时，未观察到显著相关性。然而，当考虑两者的关系时，证明了第二个独特的组，该组共享一个等位基因加上第二个等位基因的一个抗原。在这个组内，在一些家族中，即使在增强培养条件下也未观察到刺激。采用这些相同的标准，在两个等位基因均不相容的那些组中，无论匹配抗原的数量如何，或者在共享一个等位基因但在第二个等位基因的两个抗原上不同的组中，MLC反应均无显著差异。这些结果支持了编码HL - A抗原的基因座与混合培养反应之间存在密切关系的概念。它们表明，作为一个整体起作用的HL - A单倍型不相容性是MLC反应的主要刺激因素，并且单倍型的免疫原性还与刺激细胞和反应细胞是否有一个共同抗原有关。
