用于细菌检测的¹⁴CO₂放射性测量方法的灵敏度。
Sensitivity of the 14 CO 2 radiometric method for bacterial detection.
作者信息
Waters J R
出版信息
Appl Microbiol. 1972 Jan;23(1):198-9. doi: 10.1128/am.23.1.198-199.1972.
Abstract
Decimal serial dilutions of eight common bacterial species were prepared, and the detection times were determined by measuring the (14)CO(2) metabolized from the (14)C-labeled glucose substrate. The detection time was proportional to the logarithm of the initial inoculum, and high sensitivity, down to 1 colonyforming unit, was demonstrated.
摘要
制备了八种常见细菌的十进制系列稀释液，并通过测量从¹⁴C标记的葡萄糖底物代谢产生的¹⁴CO₂来确定检测时间。检测时间与初始接种量的对数成正比，并且显示出高灵敏度，低至1个菌落形成单位。
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