用于检测脱脂奶粉中沙门氏菌的24小时免疫荧光技术。
Twenty-four-hour immunofluorescence technique for the detection of salmonellae in nonfat dry milk.
作者信息
Reamer R H, Hargrove R E
出版信息
Appl Microbiol. 1972 Jan;23(1):78-81. doi: 10.1128/am.23.1.78-81.1972.
Abstract
A detection procedure was developed in which a newly devised lysine-iron medium was used as a one-step selective and enrichment medium for detection of salmonellae by the fluorescent-antibody technique. Incubation was conducted in two steps: initially at 30 C for 5 hr to resuscitate sublethally stressed cells, followed by incubation at 39 C for 17 hr. Twenty-seven strains of salmonellae from groups A-I were utilized in the development of this procedure which was sensitive enough to detect one Salmonella bacterium in 100 g of nonfat dry milk.
摘要
开发了一种检测程序，其中新设计的赖氨酸铁培养基用作一步选择性富集培养基，通过荧光抗体技术检测沙门氏菌。培养分两步进行：最初在30℃下培养5小时，以使亚致死应激细胞复苏，然后在39℃下培养17小时。在该程序的开发过程中使用了来自A-I组的27株沙门氏菌，该程序灵敏度足以检测100克脱脂奶粉中的一个沙门氏菌。
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