Campbell A, Del Campillo-Campbell A, Chang R
Proc Natl Acad Sci U S A. 1972 Mar;69(3):676-80. doi: 10.1073/pnas.69.3.676.
A recessive mutation (bir) of E. coli, at 77 min on the standard map, destroys ability of the bacteria to take up biotin or to grow except at high concentrations of biotin. In a biotin prototroph, the bir mutation causes overproduction and excretion of biotin, and derepression of at least one biosynthetic gene (bioD). Growth of the bir mutant is inhibited by avidin or high concentrations of dethiobiotin. Both effects are reversed by biotin.
大肠杆菌的一种隐性突变（bir）位于标准图谱的77分钟处，它破坏了细菌摄取生物素的能力，并且除非在高浓度生物素的情况下，细菌无法生长。在生物素原养型中，bir突变会导致生物素的过量产生和分泌，以及至少一个生物合成基因（bioD）的去阻遏。bir突变体的生长受到抗生物素蛋白或高浓度脱硫生物素的抑制。这两种效应都可被生物素逆转。
