Evans M J, Levy L
Infect Immun. 1972 Feb;5(2):238-47. doi: 10.1128/iai.5.2.238-247.1972.
Ultrastructural changes in cells of the mouse footpad are described which occurred during the log phase of multiplication, the plateau, and the stationary phase of growth of Mycobacterium leprae.BALB/c mice were inoculated in the right hind footpad with 5 x 10(3) organisms and sacrificed in pairs at 86 to 173 days after inoculation. Tissue samples were prepared for electron microscopy by standard techniques. During the early growth phase of M. leprae in the mouse footpad, few organisms can be detected. Those present are in macrophages and are bound by a single membrane. The cytoplasm of the macrophage is less dense around the organism. There are few lysosomes, and the bacteria do not appear to be degenerating. At the peak of the growth phase, the organisms within a macrophage are bound by either a single or double membrane. There is an increased number of vacuoles, which are also bound by a double membrane, and lysosomes. During the stationary phase, most of the macrophages have taken on a vacuolar appearance and contain lysosomes. The vacuoles are bound by a double membrane, as are most of the organisms within the macrophage. Many of these organisms appear to be degenerating. Occasionally, organisms are encountered in the sarcoplasm of striated muscle. They are usually bound by a single membrane and do not appear to be degenerating.
描述了麻风分枝杆菌在小鼠足垫中增殖对数期、平台期和生长静止期期间细胞的超微结构变化。将5×10³个菌接种到BALB/c小鼠的右后足垫，在接种后86至173天成对处死小鼠。通过标准技术制备用于电子显微镜检查的组织样本。在小鼠足垫中麻风分枝杆菌的早期生长阶段，可检测到的菌很少。存在的菌位于巨噬细胞内，被单层膜包绕。巨噬细胞的胞质在菌周围密度较低。溶酶体很少，且细菌似乎没有退化。在生长阶段的高峰期，巨噬细胞内的菌被单层或双层膜包绕。空泡数量增加，空泡也被双层膜包绕，还有溶酶体。在静止期，大多数巨噬细胞呈现空泡状外观并含有溶酶体。空泡被双层膜包绕，巨噬细胞内的大多数菌也是如此。许多这些菌似乎正在退化。偶尔，在横纹肌的肌浆中会遇到菌。它们通常被单层膜包绕，且似乎没有退化。
