Krisch H M, Hamlett N V, Berger H
Genetics. 1972 Oct;72(2):187-203. doi: 10.1093/genetics/72.2.187.
Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period. This is in contrast to the time course of recombination observed in wild-type, lysis-inhibited, or lysis-defective (gene t defective) infection. In all of these cases recombination under standard cross conditions occurs early in the vegetative cycle. The increased recombination in ligase-deficient rII infection is reduced in a bacterial strain which produces greater than normal levels of host ligase. These results indicate that ligase has a crucial role not only in the replication of DNA but also in recombination. The level of ligase may determine whether DNA replication occurs with or without concomitant recombination.
用连接酶缺陷型的rII噬菌体T4D感染大肠杆菌B后，连锁标记之间的重组增加了4.2倍，杂合子频率增加了2.3倍。在这种感染中，重组在较长时间内以快速速率发生。这与在野生型、裂解抑制型或裂解缺陷型（基因t缺陷型）感染中观察到的重组时间进程形成对比。在所有这些情况下，标准杂交条件下的重组在营养周期早期发生。在产生高于正常水平宿主连接酶的细菌菌株中，连接酶缺陷型rII感染中增加的重组减少了。这些结果表明，连接酶不仅在DNA复制中起关键作用，而且在重组中也起关键作用。连接酶的水平可能决定DNA复制是否伴随重组发生。
