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一种配备光散射检测的停流装置及其在生化反应中的应用。

A stopped-flow apparatus with light-scattering detection and its application to biochemical reactions.

作者信息

Riesner D, Buenemann H

出版信息

Proc Natl Acad Sci U S A. 1973 Mar;70(3):890-3. doi: 10.1073/pnas.70.3.890.

DOI:10.1073/pnas.70.3.890
PMID:4577138
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433382/
Abstract

A stopped-flow apparatus utilizing light-scattering for following the progress of a reaction is described. The method is applicable to all reactions that result in a significant change of the average molecular weight. It was possible due to several modifications of a conventional stopped-flow system to obtain a sensitivity comparable to that of commercial instruments for static light-scattering measurements. Experiments on three reactions are reported: association and dissociation of mercury ligands with DNA, dissociation of the dimers of DNA-dependent RNA polymerase, and complex formation of tRNA(Ser) (yeast) with the cognate aminoacyl-tRNA synthetase. The changes in the intensities of the scattered light are calculated and compared with the measured amplitudes.

摘要

描述了一种利用光散射跟踪反应进程的停流装置。该方法适用于所有导致平均分子量发生显著变化的反应。由于对传统停流系统进行了多项改进，从而有可能获得与商用静态光散射测量仪器相当的灵敏度。报告了三个反应的实验：汞配体与DNA的缔合和解离、依赖DNA的RNA聚合酶二聚体的解离以及酵母tRNA(Ser)与同源氨酰-tRNA合成酶的复合物形成。计算了散射光强度的变化，并与测量的振幅进行了比较。

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