经甲苯处理的大肠杆菌仅复制那些在体内即将被复制的DNA。
Toluene-treated Escherichia coli replicate only that DNA which was about to be replicated in vivo.
作者信息
Burger R M
出版信息
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2124-6. doi: 10.1073/pnas.68.9.2124.
Abstract
DNA density-transfer experiments with Escherichia coli strain B/r indicate that the DNA synthesized from exogenous substrates after treatment with toluene is made semiconservatively from that region of the chromosome about to be replicated at the time of toluene treatment. Up to 15% of the chromosome is replicated, at a rate 10-20% of that in vivo. A minor fraction of nonconservatively replicated DNA is readily distinguished.
摘要
用大肠杆菌B/r菌株进行的DNA密度转移实验表明，用甲苯处理后从外源底物合成的DNA是从甲苯处理时即将复制的染色体区域以半保留方式合成的。高达15%的染色体被复制，其速率为体内复制速率的10 - 20%。一小部分非保守复制的DNA很容易被区分出来。
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