Warr G A, Martin R R
Infect Immun. 1973 Aug;8(2):222-7. doi: 10.1128/iai.8.2.222-227.1973.
The responsiveness of alveolar macrophages lavaged from healthy volunteers to migration inhibitory factor (MIF) was tested by using the capillary-tube assay method. In every instance, macrophages from nonsmokers responded to MIF as demonstrated by a depression in migration of at least 30%, whereas MIF did not inhibit migration of macrophages from smokers. Cells from smokers migrated at a rate three times faster than cells from nonsmokers. Migration of macrophages from nonsmokers with delayed hypersensitivity skin reactions to Candida albicans antigen was inhibited when antigen was present in the tissue culture medium. Antigen did not inhibit macrophages from subjects who lacked delayed hypersensitivity to the antigen, or from subjects who were cigarette smokers. Since alveolar macrophages can respond to MIF in vitro, they may play a role in cell-mediated immune reactions in the lung. Cigarette smoking may interfere with this participation.
采用毛细管试验法检测了从健康志愿者体内灌洗出的肺泡巨噬细胞对迁移抑制因子（MIF）的反应性。在每种情况下，非吸烟者的巨噬细胞对MIF有反应，表现为迁移至少降低30%，而MIF并未抑制吸烟者巨噬细胞的迁移。吸烟者的细胞迁移速度比非吸烟者的细胞快三倍。当组织培养基中存在白色念珠菌抗原时，对该抗原具有迟发型超敏皮肤反应的非吸烟者的巨噬细胞迁移受到抑制。抗原并未抑制对该抗原缺乏迟发型超敏反应的受试者或吸烟者的巨噬细胞。由于肺泡巨噬细胞在体外可对MIF作出反应，它们可能在肺部的细胞介导免疫反应中发挥作用。吸烟可能会干扰这种参与。
