噬菌体T4的功能性基因32产物在紫外线修复中的需求。
Requirement of a functional gene 32 product of bacteriophage T4 in UV, repair.
作者信息
Wu J R, Yeh Y C
出版信息
J Virol. 1973 Oct;12(4):758-65. doi: 10.1128/JVI.12.4.758-765.1973.
Abstract
A temperature-sensitive mutation in gene 32 was used to study the role of gene 32 protein in the repair of UV-damaged DNA of bacteriophage T4. It was possible to distinguish between repair and replication of DNA at 33 C. At this temperature, DNA replication continued, and the intracellular DNA was stable. In contrast, no significant repair of UV-damaged DNA was observed even 40 min after the irradiation. Therefore, it was concluded that the defect in the repair mechanism at this temperature is not a simple consequence of the defect in DNA replication but that gene 32 apparently has an independent role for DNA repair. It was reported previously that gene 32 product is required for both T4 DNA replication and genetic recombination. In addition to these findings, this study has given direct evidence that, in vivo, this protein is also essential for the UV repair mechanism.
摘要
利用基因32中的一个温度敏感突变来研究基因32蛋白在噬菌体T4紫外线损伤DNA修复中的作用。在33℃时能够区分DNA的修复和复制。在此温度下，DNA复制继续进行，细胞内DNA稳定。相比之下，即使在照射后40分钟，也未观察到紫外线损伤DNA的显著修复。因此得出结论，在此温度下修复机制的缺陷并非DNA复制缺陷的简单结果，而是基因32显然对DNA修复具有独立作用。先前有报道称，T4 DNA复制和基因重组都需要基因32产物。除了这些发现外，本研究还提供了直接证据，证明在体内该蛋白对紫外线修复机制也是必不可少的。
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本文引用的文献
1
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J Virol. 1970 Mar;5(3):368-80. doi: 10.1128/JVI.5.3.368-380.1970.
2
Sedimentation rate as a measure of molecular weight of DNA.沉降速率作为DNA分子量的一种衡量指标。
Biophys J. 1963 Jul;3(4):309-21. doi: 10.1016/s0006-3495(63)86823-x.
3
Interpretation of sucrose gradient sedimentation pattern of deoxyribonucleic acid fragments resulting from random breaks.对随机断裂产生的脱氧核糖核酸片段蔗糖梯度沉降模式的解读。
J Virol. 1969 Jul;4(1):24-30. doi: 10.1128/JVI.4.1.24-30.1969.
4
Flexibility of native DNA from the sedimentation behavior as a function of molecular weight and temperature.天然DNA的柔韧性与其沉降行为随分子量和温度的变化关系。
J Mol Biol. 1968 Jul 14;35(1):111-29. doi: 10.1016/s0022-2836(68)80041-5.
5
T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.T4噬菌体基因32：DNA复制与重组中的一种结构蛋白。
Nature. 1970 Sep 26;227(5265):1313-8. doi: 10.1038/2271313a0.
6
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J Mol Biol. 1966 Jun;18(1):127-43. doi: 10.1016/s0022-2836(66)80081-5.
7
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J Virol. 1967 Dec;1(6):1193-202. doi: 10.1128/JVI.1.6.1193-1202.1967.
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