噬菌体phiX174 DNA在大肠杆菌C的温度敏感型dnaC突变体中的复制
Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C.
作者信息
Kranias E G, Dumas L B
出版信息
J Virol. 1974 Jan;13(1):146-54. doi: 10.1128/JVI.13.1.146-154.1974.
Abstract
Bacteriophage phiX174 cannot grow in a temperature-sensitive dnaC mutant of Escherichia coli C at the nonpermissive temperature. The inability to grow is the result of inhibition of virus DNA synthesis. Parental replicative form synthesis is not temperature sensitive. Single-stranded virus DNA continues to be synthesized for at least 45 min after shifting to the nonpermissive temperature late in infection. In contrast, the replication of the replicative form terminates within 5 min after shifting to the nonpermissive temperature.
摘要
噬菌体phiX174在非允许温度下不能在大肠杆菌C的温度敏感型dnaC突变体中生长。无法生长是病毒DNA合成受到抑制的结果。亲本复制型的合成对温度不敏感。在感染后期转移到非允许温度后，单链病毒DNA至少持续合成45分钟。相比之下，复制型的复制在转移到非允许温度后5分钟内终止。
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