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哺乳动物核糖体亚基的组成：重新评估

Composition of mammalian ribosomal subunits: a re-evaluation.

作者信息

McConkey E H

出版信息

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1379-83. doi: 10.1073/pnas.71.4.1379.

DOI:10.1073/pnas.71.4.1379
PMID:4598303
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388232/
Abstract

A method for preparation of highly active mammalian ribosomal subunits is described, which yields 60S subunits containing no more than 33% protein. It is suggested that the composition of these subunits corresponds closely to that of Escherichia coli 50S subunits. Data on the composition of bacterial and mammalian ribosomal subunits recovered from CsCl are given. It is shown that the commonly employed assumptions about the relationship between the buoyant density of a particle in CsCl and its protein content are in error. The composition of ribosomal subunits cannot ordinarily be calculated from the buoyant density in CsCl.

摘要

本文描述了一种制备高活性哺乳动物核糖体亚基的方法，该方法可得到蛋白质含量不超过33%的60S亚基。研究表明，这些亚基的组成与大肠杆菌50S亚基的组成非常接近。文中给出了从氯化铯中回收的细菌和哺乳动物核糖体亚基的组成数据。结果表明，关于颗粒在氯化铯中的浮力密度与其蛋白质含量之间关系的常用假设是错误的。核糖体亚基的组成通常不能根据其在氯化铯中的浮力密度来计算。

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本文引用的文献

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Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
2
Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.哺乳动物细胞胞质核糖核蛋白颗粒的浮力密度：核糖体亚基和快速标记成分的独特特征。
J Mol Biol. 1966 Apr;16(2):255-68. doi: 10.1016/s0022-2836(66)80171-7.
3
Molecular weights of ribosomal RNA in relation to evolution.核糖体RNA分子量与进化的关系
J Mol Biol. 1968 Dec;38(3):355-65. doi: 10.1016/0022-2836(68)90391-4.
4
Stoichiometry of the 30S ribosomal proteins of Escherichia coli.大肠杆菌30S核糖体蛋白的化学计量学
Biochemistry. 1971 Feb 2;10(3):517-24. doi: 10.1021/bi00779a026.
5
The assembly of ribosomes in yeast.酵母中核糖体的组装。
J Biol Chem. 1971 Jan 25;246(2):447-54.
6
Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.哺乳动物核糖体的结构与功能。I. 活性肝脏核糖体亚基的分离与特性分析。
J Mol Biol. 1970 Oct 14;53(1):1-19. doi: 10.1016/0022-2836(70)90042-2.
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J Mol Biol. 1969 Feb 14;39(3):545-50. doi: 10.1016/0022-2836(69)90144-2.
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The requirements for specific sRNA binding by ribosomes.核糖体对特定小RNA（sRNA）结合的要求。
J Mol Biol. 1966 Jun;18(1):90-108. doi: 10.1016/s0022-2836(66)80079-7.
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Studies on the structure of ribosomes. II. Stepwise dissociation of protein from ribosomes by caesium chloride and the re-assembly of ribosome-like particles.核糖体结构研究。II. 利用氯化铯使蛋白质逐步从核糖体上解离以及核糖体样颗粒的重新组装
J Mol Biol. 1966 Jan;15(1):268-81. doi: 10.1016/s0022-2836(66)80226-7.
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Use of formaldehyde fixation for studies of ribonucleoprotein particles by caesium chloride density-gradient centrifugation.使用甲醛固定法通过氯化铯密度梯度离心法研究核糖核蛋白颗粒。
J Mol Biol. 1965 Dec;14(2):611-5. doi: 10.1016/s0022-2836(65)80213-3.