McConkey E H
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1379-83. doi: 10.1073/pnas.71.4.1379.
A method for preparation of highly active mammalian ribosomal subunits is described, which yields 60S subunits containing no more than 33% protein. It is suggested that the composition of these subunits corresponds closely to that of Escherichia coli 50S subunits. Data on the composition of bacterial and mammalian ribosomal subunits recovered from CsCl are given. It is shown that the commonly employed assumptions about the relationship between the buoyant density of a particle in CsCl and its protein content are in error. The composition of ribosomal subunits cannot ordinarily be calculated from the buoyant density in CsCl.
本文描述了一种制备高活性哺乳动物核糖体亚基的方法,该方法可得到蛋白质含量不超过33%的60S亚基。研究表明,这些亚基的组成与大肠杆菌50S亚基的组成非常接近。文中给出了从氯化铯中回收的细菌和哺乳动物核糖体亚基的组成数据。结果表明,关于颗粒在氯化铯中的浮力密度与其蛋白质含量之间关系的常用假设是错误的。核糖体亚基的组成通常不能根据其在氯化铯中的浮力密度来计算。