哺乳动物核糖体亚基的组成：重新评估
Composition of mammalian ribosomal subunits: a re-evaluation.
作者信息
McConkey E H
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1379-83. doi: 10.1073/pnas.71.4.1379.
Abstract
A method for preparation of highly active mammalian ribosomal subunits is described, which yields 60S subunits containing no more than 33% protein. It is suggested that the composition of these subunits corresponds closely to that of Escherichia coli 50S subunits. Data on the composition of bacterial and mammalian ribosomal subunits recovered from CsCl are given. It is shown that the commonly employed assumptions about the relationship between the buoyant density of a particle in CsCl and its protein content are in error. The composition of ribosomal subunits cannot ordinarily be calculated from the buoyant density in CsCl.
摘要
本文描述了一种制备高活性哺乳动物核糖体亚基的方法，该方法可得到蛋白质含量不超过33%的60S亚基。研究表明，这些亚基的组成与大肠杆菌50S亚基的组成非常接近。文中给出了从氯化铯中回收的细菌和哺乳动物核糖体亚基的组成数据。结果表明，关于颗粒在氯化铯中的浮力密度与其蛋白质含量之间关系的常用假设是错误的。核糖体亚基的组成通常不能根据其在氯化铯中的浮力密度来计算。
相似文献
1
Composition of mammalian ribosomal subunits: a re-evaluation.哺乳动物核糖体亚基的组成：重新评估
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1379-83. doi: 10.1073/pnas.71.4.1379.
2
Mitochondrial and cytoplasmic ribosomes from mammalian tissues. Further characterization of ribosomal subunits and validity of buoyant-density methods for determination of the chemical composition and partial specific volume of ribonucleoprotein particles.哺乳动物组织中的线粒体核糖体和细胞质核糖体。核糖体亚基的进一步表征以及用于测定核糖核蛋白颗粒化学成分和偏比容的浮力密度法的有效性。
Biochem J. 1977 Nov 15;168(2):245-59. doi: 10.1042/bj1680245.
3
Particle weights and protein composition of the ribosomal subunits of the extremely thermoacidophilic archaebacterium Caldariella acidophila.嗜热嗜酸古细菌嗜酸热硫化叶菌核糖体亚基的颗粒重量和蛋白质组成
Biochem J. 1983 Feb 1;209(2):461-70. doi: 10.1042/bj2090461.
4
The structural basis for functional inactivity of reconstituted 50-S ribosomal subunits of Escherichia coli.大肠杆菌重组50-S核糖体亚基功能失活的结构基础。
Biochim Biophys Acta. 1973 Sep 7;319(3):388-400. doi: 10.1016/0005-2787(73)90179-2.
5
Molecular morphology of ribosomes. Localization of ribosomal proteins in 50-S subunits.核糖体的分子形态。核糖体蛋白在50-S亚基中的定位。
Eur J Biochem. 1973 Mar 15;33(3):481-5. doi: 10.1111/j.1432-1033.1973.tb02706.x.
6
Identification of the proteins associated with subparticles produced by mild ribonuclease digestion of 30S ribosomal particles from Escherichia coli.鉴定与大肠杆菌30S核糖体颗粒经温和核糖核酸酶消化产生的亚颗粒相关的蛋白质。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):544-8. doi: 10.1073/pnas.69.3.544.
7
Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.核糖体蛋白。8. 大肠杆菌分离核糖体蛋白的分子量。
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1909-13. doi: 10.1073/pnas.67.4.1909.
8
Size heterogeneity of the large ribosomal subunits and conservation of the small subunits in eucaryote evolution.真核生物进化过程中核糖体大亚基的大小异质性及小亚基的保守性
Biochim Biophys Acta. 1972 Nov 9;281(4):597-624. doi: 10.1016/0005-2787(72)90159-1.
9
RNA--protein interactions in ribosomes. 1. Thermal denaturation of RNA in ribosomes.核糖体中的RNA-蛋白质相互作用。1. 核糖体中RNA的热变性
Mol Biol. 1971 Jan-Feb;5(1):120-5.
10
Localization of the stringent protein of Escherichia coli on the 50S ribosomal subunit.大肠杆菌严谨反应蛋白在50S核糖体亚基上的定位
Proc Natl Acad Sci U S A. 1974 Mar;71(3):820-4. doi: 10.1073/pnas.71.3.820.
引用本文的文献
1
Impaired Ribosomal Biogenesis by Noncanonical Degradation of β-Catenin during Hyperammonemia.高血氨症中非典型β-连环蛋白降解导致核糖体生物发生受损。
Mol Cell Biol. 2019 Jul 29;39(16). doi: 10.1128/MCB.00451-18. Print 2019 Aug 15.
2
Proteomic analysis of ribosomes: translational control of mRNA populations by glycogen synthase GYS1.核糖体的蛋白质组学分析：糖原合酶 GYS1 对 mRNA 群体的翻译调控。
J Mol Biol. 2011 Jul 1;410(1):118-30. doi: 10.1016/j.jmb.2011.04.064. Epub 2011 May 5.
3
Increased transcription and decreased degradation control and recovery of liver ribosomes after a period of protein starvation.在一段蛋白质饥饿期后，肝脏核糖体的转录增加以及降解减少可控制并促进其恢复。
Biochem J. 1980 Dec 15;192(3):935-40. doi: 10.1042/bj1920935.
4
Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila.热休克诱导果蝇核糖体蛋白的快速去磷酸化。
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1781-5. doi: 10.1073/pnas.79.6.1781.
5
Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients.粗糙脉孢菌中的线粒体核糖体组装。通过在CsCl梯度中进行平衡离心对成熟和部分组装的核糖体亚基进行结构分析。
J Cell Biol. 1982 Oct;95(1):267-77. doi: 10.1083/jcb.95.1.267.
6
Differences between newly formed and recycled free small ribosome subunits in liver cytoplasm.肝脏细胞质中新形成的游离小核糖体亚基与循环利用的游离小核糖体亚基之间的差异。
Biochem J. 1976 Jul 15;158(1):97-103. doi: 10.1042/bj1580097.
7
Phosphorylation of ribosomal protein S6 in suspension cultured HeLa cells.悬浮培养的HeLa细胞中核糖体蛋白S6的磷酸化
Mol Gen Genet. 1977 Apr 29;152(3):223-30. doi: 10.1007/BF00693074.
8
Mitochondrial and cytoplasmic ribosomes from mammalian tissues. Further characterization of ribosomal subunits and validity of buoyant-density methods for determination of the chemical composition and partial specific volume of ribonucleoprotein particles.哺乳动物组织中的线粒体核糖体和细胞质核糖体。核糖体亚基的进一步表征以及用于测定核糖核蛋白颗粒化学成分和偏比容的浮力密度法的有效性。
Biochem J. 1977 Nov 15;168(2):245-59. doi: 10.1042/bj1680245.
9
Messenger ribonucleoprotein complexes isolated by oligodeoxythymidylate-cellulose chromatography from Neurospora crassa polysomes.通过寡聚脱氧胸苷酸 - 纤维素色谱法从粗糙脉孢菌多聚核糖体中分离出的信使核糖核蛋白复合物。
J Bacteriol. 1977 Jul;131(1):240-6. doi: 10.1128/jb.131.1.240-246.1977.
10
Characterisation of ribosomal proteins from HeLa and Krebs II mouse ascites tumor cells by different two-dimensional polyacrylamide gel electrophoresis techniques.采用不同的二维聚丙烯酰胺凝胶电泳技术对来自HeLa细胞和克雷布斯II型小鼠腹水瘤细胞的核糖体蛋白进行表征。
Mol Gen Genet. 1978 Apr 17;160(3):297-309. doi: 10.1007/BF00332973.
本文引用的文献
1
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
2
Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.哺乳动物细胞胞质核糖核蛋白颗粒的浮力密度：核糖体亚基和快速标记成分的独特特征。
J Mol Biol. 1966 Apr;16(2):255-68. doi: 10.1016/s0022-2836(66)80171-7.
3
Molecular weights of ribosomal RNA in relation to evolution.核糖体RNA分子量与进化的关系
J Mol Biol. 1968 Dec;38(3):355-65. doi: 10.1016/0022-2836(68)90391-4.
4
Stoichiometry of the 30S ribosomal proteins of Escherichia coli.大肠杆菌30S核糖体蛋白的化学计量学
Biochemistry. 1971 Feb 2;10(3):517-24. doi: 10.1021/bi00779a026.
5
The assembly of ribosomes in yeast.酵母中核糖体的组装。
J Biol Chem. 1971 Jan 25;246(2):447-54.
6
Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.哺乳动物核糖体的结构与功能。I. 活性肝脏核糖体亚基的分离与特性分析。
J Mol Biol. 1970 Oct 14;53(1):1-19. doi: 10.1016/0022-2836(70)90042-2.
7
Molecular weights of some HeLa ribosomal RNA's.一些海拉细胞核糖体RNA的分子量
J Mol Biol. 1969 Feb 14;39(3):545-50. doi: 10.1016/0022-2836(69)90144-2.
8
The requirements for specific sRNA binding by ribosomes.核糖体对特定小RNA（sRNA）结合的要求。
J Mol Biol. 1966 Jun;18(1):90-108. doi: 10.1016/s0022-2836(66)80079-7.
9
Studies on the structure of ribosomes. II. Stepwise dissociation of protein from ribosomes by caesium chloride and the re-assembly of ribosome-like particles.核糖体结构研究。II. 利用氯化铯使蛋白质逐步从核糖体上解离以及核糖体样颗粒的重新组装
J Mol Biol. 1966 Jan;15(1):268-81. doi: 10.1016/s0022-2836(66)80226-7.
10
Use of formaldehyde fixation for studies of ribonucleoprotein particles by caesium chloride density-gradient centrifugation.使用甲醛固定法通过氯化铯密度梯度离心法研究核糖核蛋白颗粒。
J Mol Biol. 1965 Dec;14(2):611-5. doi: 10.1016/s0022-2836(65)80213-3.
Zotero 插件