影响新型隐球菌对5-氟胞嘧啶药敏试验的变量。
Variables influencing susceptibility testing of Cryptococcus neoformans to 5-fluorocytosine.
作者信息
Block E R, Jennings A E, Bennett J E
出版信息
Antimicrob Agents Chemother. 1973 Oct;4(4):392-5. doi: 10.1128/AAC.4.4.392.
Abstract
The minimum inhibitory concentration (MIC) of 5-fluorocytosine (5-FC) was determined for 65 isolates of Cryptococcus neoformans by using a twofold serial tube dilution method. The MIC was profoundly influenced by incubation temperature, inoculum size, and duration of incubation. By using a standard set of test conditions, 100% of 49 pretreatment isolates of C. neoformans were susceptible to 10 mug of 5-FC per ml or less, and 9 (56%) of 16 isolates recovered during or after 5-FC therapy were massively drug resistant (MIC > 320 mug/ml). With the standard test conditions recommended here, the tube dilution method was found to be both accurate and reproducible, and the results correlated with the treatment status of patients.
摘要
采用二倍系列试管稀释法测定了65株新型隐球菌对5-氟胞嘧啶（5-FC）的最低抑菌浓度（MIC）。MIC受培养温度、接种量和培养时间的影响很大。采用一组标准测试条件时，49株新型隐球菌治疗前分离株中有100%对每毫升10微克或更低浓度的5-FC敏感，在5-FC治疗期间或之后分离出的16株菌株中有9株（56%）对药物高度耐药（MIC>320微克/毫升）。采用此处推荐的标准测试条件时，发现试管稀释法准确且可重复，结果与患者的治疗状况相关。
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