噬菌体感染的大肠杆菌中的磷脂酶活性。I. T4噬菌体相关磷脂酶的证明。
Phospholipase activity in bacteriophage-infected Escherichia coli. I. Demonstration of a T4 bacteriophage-associated phospholipase.
作者信息
Nelson E T, Buller C S
出版信息
J Virol. 1974 Sep;14(3):479-84. doi: 10.1128/JVI.14.3.479-484.1974.
Abstract
Phospholipase activity has been found to be associated with T4 phage and T4 ghost particles. The attachment of the phospholipase to the phage persists during purification through cesium chloride gradients and dialysis, indicating that it is firmly bound. The presence of the enzymatic activity on T4 ghosts suggests that it is not normally packaged within the head of the virus. The enzyme has specificity for phosphatidylglycerol and its activity is stimulated by 0.1% Triton X-100 and 20% methanol. It does not have a requirement for Ca(2+) and is inactivated at temperatures above 60 C. The association of the phospholipase with T4 phage grown in a phospholipase-deficient host and its absence on unsuppressed T4amtA3 suggests that it may be phage gene specific.
摘要
已发现磷脂酶活性与T4噬菌体和T4空壳颗粒有关。在通过氯化铯梯度离心和透析进行纯化的过程中，磷脂酶与噬菌体的结合持续存在，这表明它结合牢固。T4空壳颗粒上存在酶活性，这表明它通常不会被包装在病毒头部。该酶对磷脂酰甘油具有特异性，其活性受到0.1% Triton X-100和20%甲醇的刺激。它不需要Ca(2+)，并且在温度高于60℃时失活。磷脂酶与在缺乏磷脂酶的宿主中生长的T4噬菌体的关联以及在未抑制的T4amtA3上不存在该酶，表明它可能具有噬菌体基因特异性。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
LIPIDS OF SERRATIA MARCESCENS.粘质沙雷氏菌的脂质
Can J Biochem. 1964 Apr;42:461-79. doi: 10.1139/o64-054.
3
Mutations affecting the lysozyme of phage T4.影响噬菌体T4溶菌酶的突变
Cold Spring Harb Symp Quant Biol. 1961;26:25-30. doi: 10.1101/sqb.1961.026.01.007.
4
Mechanism of cell wall penetration by viruses. II. Demonstration of cyclic permeability change accompanying virus infection of Escherichia coli B cells.病毒穿透细胞壁的机制。II. 大肠杆菌B细胞受病毒感染时伴随的循环通透性变化的证明。
J Exp Med. 1955 Feb 1;101(2):151-75. doi: 10.1084/jem.101.2.151.
5
Mechanism of cell wall penetration by viruses. I. An increase in host cell permeability induced by bacteriophage infection.病毒穿透细胞壁的机制。I. 噬菌体感染诱导宿主细胞通透性增加。
J Exp Med. 1954 May 1;99(5):481-94. doi: 10.1084/jem.99.5.481.
6
Phospholipid hydrolysis in Escherichia coli infected with rapid lysis mutants of Phage T4.感染噬菌体T4快速裂解突变体的大肠杆菌中的磷脂水解作用
Virology. 1971 Feb;43(2):516-8. doi: 10.1016/0042-6822(71)90326-6.
7
The lysis mechanism of phage T4: mutants affecting lysis.噬菌体T4的裂解机制：影响裂解的突变体
Virology. 1970 Mar;40(3):719-26. doi: 10.1016/0042-6822(70)90216-3.
8
Occurrence of lysophosphatides in bacteriophage T4rII-infected Escherichia coli S-6.溶血磷脂在噬菌体T4rII感染的大肠杆菌S-6中的出现。
J Virol. 1971 Oct;8(4):437-45. doi: 10.1128/JVI.8.4.437-445.1971.
9
The effect of phage T4 infection on phospholipid hydrolysis in Escherichia coli.噬菌体T4感染对大肠杆菌中磷脂水解的影响。
Virology. 1971 Apr;44(1):94-100. doi: 10.1016/0042-6822(71)90156-5.
10
[Activation by heat of bacterial enzymes acting on phospholipids].[细菌磷脂作用酶受热激活]
C R Acad Hebd Seances Acad Sci D. 1969 Jan 6;268(1):206-9.
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