对从缺乏聚合酶I的枯草芽孢杆菌中提取的经X射线照射的DNA进行体外修复。
In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I.
作者信息
Laipis P J, Ganesan A T
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3211-4. doi: 10.1073/pnas.69.11.3211.
Abstract
DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.
摘要
从枯草芽孢杆菌DNA聚合酶I缺陷型突变体的x射线照射细胞中提取的DNA，其生物活性大大降低。这种有许多单链切口的DNA，可用纯化的DNA聚合酶I和DNA连接酶在体外修复，或用野生型枯草芽孢杆菌细胞的裂解物修复，后者可恢复生物活性并增加单链分子量。直到向缺乏DNA聚合酶I的枯草芽孢杆菌细胞裂解物中加入纯化的DNA聚合酶I或野生型细胞的裂解物，这种缺陷型细胞的裂解物才能修复此类经照射的DNA。
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