Youngs D A, Smith K C
Mol Gen Genet. 1977 Mar 28;152(1):37-41. doi: 10.1007/BF00264937.
The effect of the ligts-7 mutation on cell survival and the extent of DNA repair after UV (254 nm) irradiation was determined for wild-type and uvrB5 cells of E. coli K-12 at 30 degrees and 42 degrees C. At the restrictive temperature (42 degrees C) the ligts-7 mutation resulted in (i) a decrease in the extent of repair of DNA incision breaks arising during the excision repair process, and (ii) a decrease in the extent of post-replicational repair of gaps in newly-synthesized DNA. These deficiencies in DNA repair correlated with increases in cellular sensitivity to killing by UV radiation. Thus, DNA lagase plays an important role in vivo in both the excision and post-replicational repair processes.
在30摄氏度和42摄氏度下，测定了大肠杆菌K-12野生型和uvrB5细胞中ligts-7突变对紫外线（254纳米）照射后细胞存活及DNA修复程度的影响。在限制温度（42摄氏度）下，ligts-7突变导致：（i）切除修复过程中产生的DNA切口断裂的修复程度降低；（ii）新合成DNA中缺口的复制后修复程度降低。这些DNA修复缺陷与细胞对紫外线杀伤的敏感性增加相关。因此，DNA连接酶在体内的切除修复和复制后修复过程中均发挥重要作用。
