环形试验：一种评估大麻对小鼠“僵住症”效应的定量方法。
The ring test: a quantitative method for assessing the 'cataleptic' effect of cannabis in mice.
作者信息
Pertwee R G
出版信息
Br J Pharmacol. 1972 Dec;46(4):753-63. doi: 10.1111/j.1476-5381.1972.tb06900.x.
Abstract
- A bioassay for cannabis, called the ring test, has been developed in which the percentage of the total time spent on a horizontal wire ring during which a mouse remains completely immobile is recorded.2. The effect of cannabis on mobility is a dose-related, graded response.3. Threshold doses of cannabis extract are 12.5 mg/kg when injected intravenously, and 100 mg/kg when injected intraperitoneally or subcutaneously.4. The method provides a measure of the ;cataleptic' effect of cannabis. Chlorpromazine in doses of 1 mg/kg upwards also produces the effect but barbitone does not.5. It is concluded that Delta(1)-tetrahydrocannabinol (Delta(1)-THC) is largely responsible for the effect of cannabis extract on mobility; the potency ratio of Delta(1)-THC to cannabis extract is between 10 and 20. Delta(1)-Tetrahydrocannabidivarol (Delta(1)-THD) also affects mobility but is less active than Delta(1)-THC. Cannabidiol has no effect when injected intraperitoneally in doses up to 100 mg/kg.
摘要
已开发出一种用于大麻的生物测定法，称为环形试验，该试验记录小鼠在水平金属丝环上完全静止不动的总时间百分比。
大麻对活动能力的影响是一种与剂量相关的分级反应。
大麻提取物的阈剂量静脉注射时为12.5毫克/千克，腹腔注射或皮下注射时为100毫克/千克。
该方法可衡量大麻的“僵住”效应。剂量为1毫克/千克及以上的氯丙嗪也会产生这种效应，但巴比妥则不会。
得出的结论是，Δ(1)-四氢大麻酚（Δ(1)-THC）在很大程度上导致了大麻提取物对活动能力的影响；Δ(1)-THC与大麻提取物的效价比在10至20之间。Δ(1)-四氢大麻二酚（Δ(1)-THD）也会影响活动能力，但活性低于Δ(1)-THC。腹腔注射剂量高达100毫克/千克的大麻二酚没有效果。
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