通过酶联免疫吸附测定法检测肺支原体抗体。
Detection of antibodies to Mycoplasma pulmonis by an enzyme-linked immunosorbent assay.
作者信息
Horowitz S A, Cassell G H
出版信息
Infect Immun. 1978 Oct;22(1):161-70. doi: 10.1128/iai.22.1.161-170.1978.
Abstract
The enzyme-linked immunosorbent assay, which entails the use of antigen-coated tubes and enzyme-labeled anti-immunoglobulins, was applied for the detection of antibodies against Mycoplasma pulmonis in mice. A lysate of M. pulmonis was used as the antigen, and anti-mycoplasmal antibodies of the different immunoglobulin classes were detected by class-specific anti-immunoglobulin labeled with alkaline phosphatase. The optimal conditions for the test were determined, the specificity was evaluated, and the assay was compared with other procedures for detection of mycoplasmal infection. The enzyme-linked immunosorbent assay was found to be a specific, highly sensitive, and reliable procedure for detecting anti-mycoplasmal antibodies in mice.
摘要
酶联免疫吸附测定法，该方法需要使用包被抗原的试管和酶标记的抗免疫球蛋白，用于检测小鼠抗肺支原体抗体。用肺支原体裂解物作为抗原，通过用碱性磷酸酶标记的类特异性抗免疫球蛋白检测不同免疫球蛋白类别的抗支原体抗体。确定了该试验的最佳条件，评估了特异性，并将该测定法与检测支原体感染的其他方法进行了比较。发现酶联免疫吸附测定法是检测小鼠抗支原体抗体的一种特异性强、灵敏度高且可靠的方法。
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