胸腺细胞的GIX抗原与鼠白血病病毒包膜糖蛋白的关系。
Relation of GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus.
作者信息
Obata Y, Ikeda H, Stockert E, Boyse E A
出版信息
J Exp Med. 1975 Jan 1;141(1):188-97. doi: 10.1084/jem.141.1.188.
Expression of Gix surface antigen on thymocytes is an inherited mendelian train of certain strains of mice. We report here the following new findings: (a) Gix antigen was found free in the serum of Gix+ mouse strains. (b) Expression vs. nonexpression of Gix antigen was invariably correlated with presence or absence of the group-specific antigen of Murine leukemia virus (MuLV) gp69/71 in the serum of mice of inbred and segregating populations. (c) Gix antigen could be removed from normal Gix+ mouse serum by precipitation with antiserum to MuLV gp 69/71. (d) Anti-gp69/71 serum was weakly cytotoxic for Gix+ thymocytes, and partially blocked the cytotoxic activity of Gix antibody for Gix+ thymocytes. (e) Purified AKR virus absorbed Gix activity, and disruption of the virions did not increase their absorbing capacity. These serological data indicate that Gix antigen is a constituent of gp69/71, the glycoprotein which is the major component of the MuLV envelope. On present evidence, Gix antigen is represented in intact virions and is probably accessible to Gix antibody.
胸腺细胞上Gix表面抗原的表达是某些品系小鼠的一种孟德尔遗传性状。我们在此报告以下新发现：(a) 在Gix+小鼠品系的血清中发现了游离的Gix抗原。(b) Gix抗原的表达与否与近交和分离群体小鼠血清中鼠白血病病毒（MuLV）gp69/71的群特异性抗原的有无始终相关。(c) 通过用抗MuLV gp 69/71抗血清沉淀，可以从正常的Gix+小鼠血清中去除Gix抗原。(d) 抗gp69/71血清对Gix+胸腺细胞具有弱细胞毒性，并部分阻断了Gix抗体对Gix+胸腺细胞的细胞毒活性。(e) 纯化的AKR病毒吸收了Gix活性，病毒粒子的破坏并未增加其吸收能力。这些血清学数据表明，Gix抗原是gp69/71的一个组成部分，gp69/71是MuLV包膜的主要成分糖蛋白。根据目前的证据，Gix抗原存在于完整的病毒粒子中，并且可能可被Gix抗体识别。
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