小鼠白血病病毒包膜糖蛋白gp69/71在小鼠胸腺细胞上的表达。存在与不存在GIX抗原所区分的两种结构变体的证据。
Expression of murine leukemia virus envelope glycoprotein gp69/71 on mouse thymocytes. Evidence for two structural variants distinguished by presence vs. absence of GIX antigen.
作者信息
Tung J S, Fleissner E, Vitetta E S, Boyse E A
出版信息
J Exp Med. 1975 Aug 1;142(2):518-23. doi: 10.1084/jem.142.2.518.
Abstract
Thymocytes of several mouse strains were tested for expression of the gp69/71 envelope component of murine leukemia virus by surface iodination, followed by immunoprecipitation and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Theses strains included two congenic lines differing from their partner stocks with respect to expression of GIX antigen demonstrable in the cytoxicity assay. We conclude that:(a) two structural variants of gp69/71 can be expressed on mouse thymocytes, (b) these are distinguishable by a small difference in mobility in SDS gels, (c) one carries GIX antigen and the other not, (d) they are coded, or their expression is regulated, by different chromosomal loci that are not closely linked, and (e) both can be expressed together on the thymocytes of inbred mice. In the intact thymocyte plasma membrane, the sites of group-specific antigen shared by the two gp69/71 variants, unlike the GIX type specificity carried by only one of them, are probably inaccessible to antibody.
摘要
通过表面碘化，随后进行免疫沉淀和十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳，对几种小鼠品系的胸腺细胞进行了鼠白血病病毒包膜成分gp69/71表达的检测。这些品系包括两个同基因系，它们与其亲本品系在细胞毒性试验中可证明的GIX抗原表达方面存在差异。我们得出以下结论：（a）gp69/71的两种结构变体可在小鼠胸腺细胞上表达；（b）它们在SDS凝胶中的迁移率略有差异，以此可区分；（c）一种携带GIX抗原，另一种不携带；（d）它们由不紧密连锁的不同染色体位点编码，或其表达受其调控；（e）两者可在近交系小鼠的胸腺细胞上共同表达。在完整的胸腺细胞质膜中，两种gp69/71变体共有的群特异性抗原位点，与仅其中一种携带的GIX型特异性不同，可能无法被抗体识别。
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7
Biochemical evidence linking the GIX thymocyte surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus.将GIX胸腺细胞表面抗原与鼠白血病病毒的gp69/71包膜糖蛋白联系起来的生化证据。
J Exp Med. 1975 Jan 1;141(1):198-205. doi: 10.1084/jem.141.1.198.
8
Relation of GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus.胸腺细胞的GIX抗原与鼠白血病病毒包膜糖蛋白的关系。
J Exp Med. 1975 Jan 1;141(1):188-97. doi: 10.1084/jem.141.1.188.
9
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J Exp Med. 1975 Jan 1;141(1):172-87. doi: 10.1084/jem.141.1.172.
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