Smith J B, Ingerman C, Kocsis J J, Silver M J
J Clin Invest. 1973 Apr;52(4):965-9. doi: 10.1172/JCI107262.
Prostaglandins E(2) and F(2alpha) were formed in response to ADP, L-epinephrine, or collagen by human platelets suspended in plasma containing citrate anticoagulant and stirred at 37 degrees C. The prostaglandins formed by platelets in response to collagen were rapidly released and the amounts formed were proportional to the amount of collagen added. The formation of the prostaglandins was associated with the single wave of aggregation induced by collagen or the second wave of aggregation induced by epinephrine. The above findings are discussed with reference to published studies on the biochemical changes occurring during platelet aggregation. It is suggested that the formation and release of prostaglandins is associated with the secretion of endogenous ADP and 5-hydroxytryptamine.
悬浮于含柠檬酸盐抗凝剂的血浆中并在37℃搅拌的人血小板，在二磷酸腺苷（ADP）、L - 肾上腺素或胶原作用下会生成前列腺素E(2)和F(2α)。血小板对胶原产生反应而形成的前列腺素会迅速释放，其生成量与添加的胶原量成正比。前列腺素的形成与胶原诱导的单波聚集或肾上腺素诱导的第二波聚集有关。结合已发表的关于血小板聚集过程中发生的生化变化的研究，对上述发现进行了讨论。有人提出，前列腺素的形成和释放与内源性ADP和5 - 羟色胺的分泌有关。
