Buchet J P, Lauwerys R R
Br J Ind Med. 1973 Apr;30(2):125-8. doi: 10.1136/oem.30.2.125.
125-128. A gas chromatographic method for the determination of hippuric and m-methylhippuric acids in urine is described. A known amount of heptadecanoic acid (internal standard) is added to the urine before its extraction with ethyl acetate. After evaporation of the solvent, the acids are methylated with diazomethane. The residue is taken up in methanol and injected into the gas chromatograph. The ratio of the height of the hippuric or m-methylhippuric acid peak to the height of the heptadecanoic acid peak is calculated and by reference to a calibration curve prepared in the same conditions the urine concentration of the two acids is determined.
125 - 128. 描述了一种用于测定尿液中马尿酸和间甲基马尿酸的气相色谱法。在尿液用乙酸乙酯萃取之前，加入已知量的十七烷酸（内标）。溶剂蒸发后，酸用重氮甲烷进行甲基化。残留物用甲醇溶解并注入气相色谱仪。计算马尿酸或间甲基马尿酸峰高与十七烷酸峰高的比值，并参照在相同条件下制备的校准曲线确定两种酸在尿液中的浓度。
