Sullivan J, Geren C R
Prep Biochem. 1979;9(3):321-33. doi: 10.1080/00327487908061695.
A rapid and convenient method for the purification of a toxin from timber rattlesnake, Crotalus horridus horridus, venom using carboxymethyl cellulose ion-exchange chromatography has been devised. The toxicity of this venom component is labile, but it is stabilized by the addition of 20+ V/V glycerol to the buffer solution. This toxin has a molecular weight of 15,000 +/- 700 as determined by SDS gel electrophoresis. It is both heat and protease resistant. Treatment of this venom component with 2-mercaptoethanol followed by G-50 Sephadex chromatography causes no loss of toxicity although incubation of the toxin with 1% SDS and 1% 2-mercaptoethanol prior to electrophoresis does result in a faster migrating species. The toxin does not affect neuromuscular junctions but does appear to act on the nervous system. It causes no local responses in mice.
已设计出一种快速便捷的方法，利用羧甲基纤维素离子交换色谱法从木纹响尾蛇（Crotalus horridus horridus）毒液中纯化一种毒素。这种毒液成分的毒性不稳定，但通过向缓冲溶液中添加20%（V/V）甘油可使其稳定。通过SDS凝胶电泳测定，这种毒素的分子量为15,000±700。它既耐热又耐蛋白酶。用2-巯基乙醇处理该毒液成分，然后进行G-50葡聚糖凝胶色谱分离，毒性不会丧失，尽管在电泳前将毒素与1% SDS和1% 2-巯基乙醇一起孵育确实会产生迁移速度更快的条带。该毒素不影响神经肌肉接头，但似乎作用于神经系统。它在小鼠中不会引起局部反应。
