Jay A W
Biophys J. 1973 Nov;13(11):1166-82. doi: 10.1016/S0006-3495(73)86053-9.
Single human red blood cells suspended in buffered Ringer's solution were rapidly drawn, at recorded pressures, into glass micropipettes of diameter 0.6-3.2 mum. Cells could enter micropipettes of diameter >/= 2.9 mum with minimal pressure. In micropipettes of 0.9-2.9 mum, the pressure required increased linearly with decreasing diameter. For diameters 2.5-2.9 mum, pressures ranged up to 7 cm Hg, and the cells returned to normal biconcave shape on release. For diameters 1.9-2.5 mum, the required pressures ranged from 7 to 17 cm Hg. The released cells were crenated. In micropipettes 0.9-1.9 mum, the pressures required ranged from 17 to 34 cm Hg. The cells hemolyzed on entry. As diameter decreased from 0.9 to 0.6 mum, cells were drawn into dumbbell shapes and parts of the cells were pinched off without complete hemolysis of the cell. Using an accepted value of 138 mum(2) for the mean cell area, the mean volume of the human red cell was calculated to be 94 mum(3). Under mechanical stress, about 12% of this volume is rapidly exchangeable with the external medium. The cell volume may further decrease by 20% which is not reversible.
将悬浮于缓冲林格氏液中的单个人类红细胞，在记录的压力下迅速吸入直径为0.6 - 3.2微米的玻璃微量移液器中。细胞能够以最小压力进入直径≥2.9微米的微量移液器。在直径为0.9 - 2.9微米的微量移液器中，所需压力随直径减小呈线性增加。对于直径2.5 - 2.9微米的情况，压力可达7厘米汞柱，且细胞在释放后恢复正常双凹形。对于直径1.9 - 2.5微米的情况，所需压力范围为7至17厘米汞柱。释放后的细胞呈皱缩状。在直径0.9 - 1.9微米的微量移液器中，所需压力范围为17至34厘米汞柱。细胞在进入时发生溶血。当直径从0.9微米减小到0.6微米时，细胞被拉成哑铃状，部分细胞被挤掉，细胞未完全溶血。使用公认的平均细胞面积值138微米²，计算得出人类红细胞的平均体积为94微米³。在机械应力下，该体积的约12%可迅速与外部介质交换。细胞体积可能进一步减小20%，且这一减小是不可逆的。
