Swanson C H, Solomon A K
J Gen Physiol. 1973 Oct;62(4):407-29. doi: 10.1085/jgp.62.4.407.
Micropuncture techniques have been used to examine electrolyte secretion by the in vitro rabbit pancreas. The concentration profiles of the major secreted ions and digestive protein and the electrical potential profile within the pancreatic ductal system have been determined during spontaneous and secretin-stimulated secretion. The active transport of both Na and HCO(3) are the rate-controlling steps in primary secretion. Spontaneous secretion is produced primarily within the intralobular ducts. The anion composition of this primary secretion depends on the secretion rate with HCO(3) ranging from about 70 meq/liter at low rates to about 110 meq/liter at high rates. With secretin stimulation the smaller extralobular ducts also secrete and this extralobular secretion has a higher HCO(2) content than that of the intralobular secretion. In the main collecting duct the anion composition of the juice is modified further by Cl-HCO(3) exchange which appears to be a passive process depending on the average residence time of the juice in the main duct.
微穿刺技术已被用于研究体外兔胰腺的电解质分泌。在自发分泌和促胰液素刺激分泌过程中，已测定了胰腺导管系统内主要分泌离子、消化蛋白的浓度分布以及电势分布。钠和碳酸氢根的主动转运是初级分泌中的速率控制步骤。自发分泌主要在小叶内导管产生。这种初级分泌的阴离子组成取决于分泌速率，碳酸氢根的浓度范围从低速率时的约70毫当量/升至高速率时的约110毫当量/升。在促胰液素刺激下，较小的小叶外导管也会分泌，并且这种小叶外分泌的碳酸氢根含量高于小叶内分泌。在主集合管中，胰液的阴离子组成通过氯-碳酸氢根交换进一步改变，这似乎是一个被动过程，取决于胰液在主导管中的平均停留时间。
