Cardinali D P, Freire F
Mol Cell Endocrinol. 1975 May;2(5):317-30. doi: 10.1016/0303-7207(75)90019-2.
[3-H]Melatonin administered in vivo in the rat cisterna magna became associated with a vinblastine-precipitable protein. Melatonin treatment decreased microtubule protein content by 44% in the arcuate-median eminence region and by 19% in the remaining hypothalamic block, being without significant effect on the cerebral cortex. Superior cervical gangliectomy but not pinealectomy increased microtubule protein content of the rat hypothalamus. Norepinephrine brought about a significantly greater decrease in hypothalamic microtubule protein levels of ganglionectomized rats than in sham-operated or in ganglionectomized-pinealectomized animals. Melatonin treatment induced in most of the axons ending in the pericapillary zone of the rat median eminence crystaloid and tubular formations. Rapid axonal transport in retinal ganglion cells of rabbits was inhibited to the extent of 71.9 and 87.2% by previous exposure to 1.5 of 15 mu g of melatonin intravitreally; melatonin did not affect retinal protein synthesis in this experimental model. These results suggest that melatonin interacts significantly with microtubule or actin-like protein in brain.
大鼠脑池内注射的[3 - H]褪黑素与一种可被长春碱沉淀的蛋白质相关。褪黑素处理使弓状 - 正中隆起区域的微管蛋白含量降低了44%，在其余下丘脑区域降低了19%，而对大脑皮层无显著影响。颈上神经节切除术而非松果体切除术增加了大鼠下丘脑的微管蛋白含量。去甲肾上腺素导致神经节切除大鼠下丘脑微管蛋白水平的降低幅度显著大于假手术组或神经节切除 - 松果体切除的动物。褪黑素处理在大多数终止于大鼠正中隆起毛细血管周围区域的轴突中诱导形成晶体状和管状结构。兔视网膜神经节细胞中的快速轴突运输在玻璃体内预先暴露于1.5或15μg褪黑素后分别被抑制了71.9%和87.2%；在该实验模型中，褪黑素不影响视网膜蛋白质合成。这些结果表明，褪黑素在大脑中与微管或肌动蛋白样蛋白有显著相互作用。
