Hauptfeld V, Hauptfeld M, Klein J
J Exp Med. 1975 May 1;141(5):1047-56. doi: 10.1084/jem.141.5.1047.
Mouse spleen or thymus lymphocytes incubated with monospecific H-2 or Ia alloantisera and then coated with a xenogeneic antimouse Ig serum become specifically resistant to the alloantiserum (and complement) they have been incubated with. This so called "lysostrip method" was used to investigate the molecular interrelationships of antigens in the mouse lymphocyte membrane. The results of this investigation confirm that H-2K and H-2D antigens are carried by two distinct populations of molecules. They provide evidence that the Ia antigens move in the membrane independently of both H2-K and H-2D antigens; and finally they demonstrate absence of any physical linkage between Ig receptors in B cells, on the one hand, and Ia, H-2K, and H-2D molecules on the other hand.
将小鼠脾脏或胸腺淋巴细胞与单特异性H-2或Ia同种异体抗血清一起孵育,然后用异种抗小鼠Ig血清包被,这些细胞会对它们所孵育的同种异体抗血清(和补体)产生特异性抗性。这种所谓的“溶菌去除法”被用于研究小鼠淋巴细胞膜中抗原的分子相互关系。该研究结果证实,H-2K和H-2D抗原由两个不同的分子群体携带。它们提供了证据表明Ia抗原在膜中独立于H2-K和H-2D抗原移动;最后,它们证明了一方面B细胞中的Ig受体与另一方面的Ia、H-2K和H-2D分子之间不存在任何物理联系。