Kang Beom Ryong, Anderson Anne J, Kim Young Cheol
Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju 61186, Korea.
Department of Biology, Utah State University, Logan, UT 84322-5305, USA.
Plant Pathol J. 2018 Feb;34(1):35-43. doi: 10.5423/PPJ.OA.06.2017.0115. Epub 2018 Feb 1.
Root-knot nematodes ( spp.) are parasites that attack many field crops and orchard trees, and affect both the quantity and quality of the products. A root-colonizing bacterium, O6, possesses beneficial traits including strong nematicidal activity. To determine the molecular mechanisms involved in the nematicidal activity of O6, we constructed two mutants; one lacking hydrogen cyanide production, and a second lacking an insecticidal toxin, FitD. Root drenching with wild-type O6 cells caused juvenile mortality and . Efficacy was not altered in the mutant compared to the wild-type but was reduced in both bioassays for the mutant lacking hydrogen cyanide production. The reduced number of galls on tomato plants caused by the wild-type strain was comparable to that of a standard chemical nematicide. These findings suggest that hydrogen cyanide-producing root colonizers, such as O6, could be formulated as "green" nematicides that are compatible with many crops and offer agricultural sustainability.
根结线虫（Meloidogyne spp.）是一种寄生于多种大田作物和果园树木的寄生虫，会影响农产品的产量和质量。一种定殖于根部的细菌O6具有包括强杀线虫活性在内的有益特性。为了确定参与O6杀线虫活性的分子机制，我们构建了两个突变体；一个缺乏氰化氢生成能力，另一个缺乏杀虫毒素FitD。用野生型O6细胞进行根灌导致幼虫死亡。与野生型相比，ΔFitD突变体的效果没有改变，但在两种生物测定中，缺乏氰化氢生成能力的突变体的效果均降低。野生型菌株导致番茄植株上的虫瘿数量减少，这与标准化学杀线虫剂相当。这些发现表明，诸如O6之类的能产生氰化氢的根部定殖菌可以被配制成与许多作物相容并提供农业可持续性的“绿色”杀线虫剂。
