胰岛素对大鼠附睾脂肪组织中丙酮酸激酶活性的急性调节。
Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulin.
作者信息
Denton R M, Edgell N J, Bridges B J, Poole G P
出版信息
Biochem J. 1979 Jun 15;180(3):523-31. doi: 10.1042/bj1800523.
Abstract
- Evidence is presented that exposure of epididymal fat-pads from fed rats to insulin leads to a marked diminution in the Km for phosphoenolpyruvate of pyruvate kinase. Effects of insulin may be readily demonstrated in experiments both in vivo and in vitro and are not secondary to the activation by the hormone of glucose transport. No effect of insulin is apparent in tissues from 48 h-starved animals. 2. The mechanism of the effect of insulin on pyruvate kinase was not established. The observed changes in Km do not appear to be the result of alterations in the amounts of bound effectors such as fructose 1,6-bisphosphate and alanine. Rather, as the effect persists in incubated extracts, it appears that a change in the degree of phosphorylation or some other covalent modification of the enzyme may be involved.
摘要
- 有证据表明，喂食大鼠的附睾脂肪垫暴露于胰岛素会导致丙酮酸激酶对磷酸烯醇丙酮酸的米氏常数显著降低。胰岛素的作用在体内和体外实验中都很容易得到证明，且并非继发于该激素对葡萄糖转运的激活作用。在饥饿48小时的动物组织中，胰岛素没有明显作用。2. 胰岛素对丙酮酸激酶作用的机制尚未明确。观察到的米氏常数变化似乎不是由诸如1,6-二磷酸果糖和丙氨酸等结合效应物数量的改变所致。相反，由于这种作用在孵育提取物中持续存在，似乎可能涉及酶的磷酸化程度变化或其他一些共价修饰。
相似文献
1
Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulin.胰岛素对大鼠附睾脂肪组织中丙酮酸激酶活性的急性调节。
Biochem J. 1979 Jun 15;180(3):523-31. doi: 10.1042/bj1800523.
2
Effects of protein phosphatase inhibitors on the regulation of insulin-sensitive enzymes within rat epididymal fat-pads and cells.蛋白磷酸酶抑制剂对大鼠附睾脂肪垫及细胞内胰岛素敏感性酶调节的影响。
Biochem J. 1991 Jun 15;276 ( Pt 3)(Pt 3):649-54. doi: 10.1042/bj2760649.
3
Mechanisms regulating adipose-tissue pyruvate dehydrogenase.调节脂肪组织丙酮酸脱氢酶的机制
Biochem J. 1972 Sep;129(3):763-73. doi: 10.1042/bj1290763.
4
Regulation of pyruvate dehydrogenase activity in rat epididymal fat-pads and isolated adipocytes by adrenaline.肾上腺素对大鼠附睾脂肪垫和分离脂肪细胞中丙酮酸脱氢酶活性的调节
Biochem J. 1978 Jul 15;174(1):119-30. doi: 10.1042/bj1740119.
5
Regulation of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads. Effects of starvation, alloxan-diabetes and high-fat diet.大鼠附睾脂肪垫中丙酮酸脱氢酶及丙酮酸脱氢酶磷酸磷酸酶活性的调节。饥饿、四氧嘧啶糖尿病及高脂饮食的影响。
Biochem J. 1976 Jan 15;154(1):225-36. doi: 10.1042/bj1540225.
6
Modulation of the phosphorylation state of rat liver pyruvate kinase by allosteric effectors and insulin.变构效应剂和胰岛素对大鼠肝脏丙酮酸激酶磷酸化状态的调节
J Biol Chem. 1979 Aug 25;254(16):7855-64.
7
The role of phosphorylation in the regulation of fatty acid synthesis by insulin and other hormones.磷酸化在胰岛素及其他激素对脂肪酸合成的调节中的作用。
Philos Trans R Soc Lond B Biol Sci. 1983 Jul 5;302(1108):33-45. doi: 10.1098/rstb.1983.0036.
8
Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones.胰岛素及其他激素对脂肪组织丙酮酸脱氢酶的调节作用。
Biochem J. 1971 Nov;125(1):115-27. doi: 10.1042/bj1250115.
9
The effect of dietary and hormonal conditions on the activities of glycolytic enzymes in rat epididymal adipose tissue.饮食和激素条件对大鼠附睾脂肪组织中糖酵解酶活性的影响。
Biochem J. 1969 Nov;115(3):405-17. doi: 10.1042/bj1150405.
10
The regulation of triglyceride synthesis and fatty acid synthesis in rat epididymal adipose tissue.大鼠附睾脂肪组织中甘油三酯合成与脂肪酸合成的调节
Biochem J. 1970 Sep;119(2):193-219. doi: 10.1042/bj1190193.
引用本文的文献
1
The rôle of mitochondrial pyruvate transport in the stimulation by glucagon and phenylephrine of gluconeogenesis from L-lactate in isolated rat hepatocytes.线粒体丙酮酸转运在胰高血糖素和去氧肾上腺素刺激离体大鼠肝细胞由L-乳酸进行糖异生过程中的作用。
Biochem J. 1981 Sep 15;198(3):551-60. doi: 10.1042/bj1980551.
2
Regulation of glycolysis and fatty acid synthesis from glucose in sheep adipose tissue.绵羊脂肪组织中葡萄糖的糖酵解和脂肪酸合成的调控
Biochem J. 1982 Sep 15;206(3):577-86. doi: 10.1042/bj2060577.
3
A partial view of the mechanism of insulin action.胰岛素作用机制的部分观点。
Diabetologia. 1981 Oct;21(4):347-62. doi: 10.1007/BF00252681.
4
Rapid modulation of adipocyte phosphofructokinase activity by noradrenaline and insulin.去甲肾上腺素和胰岛素对脂肪细胞磷酸果糖激酶活性的快速调节
Biochem J. 1982 Mar 15;202(3):753-8. doi: 10.1042/bj2020753.
5
Interactions of insulin and dexamethasone in the control of pyruvate kinase activity and glucose metabolism in sheep adipose tissue.胰岛素与地塞米松在调控绵羊脂肪组织丙酮酸激酶活性及葡萄糖代谢中的相互作用
Biochem J. 1987 Oct 15;247(2):459-65. doi: 10.1042/bj2470459.
6
7
Regulation of fructose 2,6-bisphosphate concentration in white adipose tissue.白色脂肪组织中果糖-2,6-二磷酸浓度的调节
Biochem J. 1985 Jan 15;225(2):421-8. doi: 10.1042/bj2250421.
8
Effect of nutritional status on insulin sensitivity in vivo and tissue enzyme activities in the rat.营养状况对大鼠体内胰岛素敏感性及组织酶活性的影响。
Biochem J. 1989 Mar 15;258(3):699-707. doi: 10.1042/bj2580699.
本文引用的文献
1
Guinea-pig anti-insulin serum.豚鼠抗胰岛素血清。
J Physiol. 1961 Feb;155(2):302-10. doi: 10.1113/jphysiol.1961.sp006628.
2
Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones.胰岛素及其他激素对脂肪组织丙酮酸脱氢酶的调节作用。
Biochem J. 1971 Nov;125(1):115-27. doi: 10.1042/bj1250115.
3
Adipose-tissue pyruvate kinase. Properties and interconversion of two active forms.脂肪组织丙酮酸激酶。两种活性形式的性质与相互转化
Biochem J. 1968 Nov;110(1):67-77. doi: 10.1042/bj1100067.
4
Two interconvertible forms of pyruvate kinase in adipose tissue.脂肪组织中丙酮酸激酶的两种可相互转化的形式。
Biochem Biophys Res Commun. 1968 Feb 15;30(3):297-302. doi: 10.1016/0006-291x(68)90450-6.
5
Effects of L-alanine and fructose (1,6-diphosphate) on pyruvate kinase from ehrlich ascites tumour cells.L-丙氨酸和果糖（1,6-二磷酸）对艾氏腹水瘤细胞丙酮酸激酶的影响。
FEBS Lett. 1973 Nov 1;36(3):305-8. doi: 10.1016/0014-5793(73)80397-7.
6
Allosteric properties of a pyruvate kinase isoenzyme from rat liver cells in culture.
J Biol Chem. 1973 Jul 10;248(13):4610-6.
7
Kinetic evidence for the presence of two forms of M2-type pyruvate kinase in rat small intestine.
Biochem Biophys Res Commun. 1974 Sep 9;60(1):398-405. doi: 10.1016/0006-291x(74)90218-6.
8
Interconvertible and noninterconvertible forms of rat pyruvate kinase.
Biochemistry. 1972 Nov 21;11(24):4442-50. doi: 10.1021/bi00774a004.
9
Multimolecular forms of pyruvate kinase from rat and other mammalian tissues. I. Electrophoretic studies.大鼠及其他哺乳动物组织中丙酮酸激酶的多分子形式。I. 电泳研究。
J Biochem. 1972 Jun;71(6):1043-51. doi: 10.1093/oxfordjournals.jbchem.a129852.
10
Alanine-mediated reversible inactivation of tumour pyruvate kinase caused by a tetramer-dimer transition.
FEBS Lett. 1975 Feb 15;50(3):346-50. doi: 10.1016/0014-5793(75)80524-2.
Zotero 插件