胰岛素对大鼠附睾脂肪组织中丙酮酸激酶活性的急性调节。
Acute regulation of pyruvate kinase activity in rat epididymal adipose tissue by insulin.
作者信息
Denton R M, Edgell N J, Bridges B J, Poole G P
出版信息
Biochem J. 1979 Jun 15;180(3):523-31. doi: 10.1042/bj1800523.
Abstract
- Evidence is presented that exposure of epididymal fat-pads from fed rats to insulin leads to a marked diminution in the Km for phosphoenolpyruvate of pyruvate kinase. Effects of insulin may be readily demonstrated in experiments both in vivo and in vitro and are not secondary to the activation by the hormone of glucose transport. No effect of insulin is apparent in tissues from 48 h-starved animals. 2. The mechanism of the effect of insulin on pyruvate kinase was not established. The observed changes in Km do not appear to be the result of alterations in the amounts of bound effectors such as fructose 1,6-bisphosphate and alanine. Rather, as the effect persists in incubated extracts, it appears that a change in the degree of phosphorylation or some other covalent modification of the enzyme may be involved.
摘要
- 有证据表明，喂食大鼠的附睾脂肪垫暴露于胰岛素会导致丙酮酸激酶对磷酸烯醇丙酮酸的米氏常数显著降低。胰岛素的作用在体内和体外实验中都很容易得到证明，且并非继发于该激素对葡萄糖转运的激活作用。在饥饿48小时的动物组织中，胰岛素没有明显作用。2. 胰岛素对丙酮酸激酶作用的机制尚未明确。观察到的米氏常数变化似乎不是由诸如1,6-二磷酸果糖和丙氨酸等结合效应物数量的改变所致。相反，由于这种作用在孵育提取物中持续存在，似乎可能涉及酶的磷酸化程度变化或其他一些共价修饰。
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Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones.胰岛素及其他激素对脂肪组织丙酮酸脱氢酶的调节作用。
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