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大肠杆菌K-12的D-丝氨酸脱氨酶系统中稳定部分二倍体的显性研究。

Dominance studies with stable merodiploids in the D-serine deaminase system of Escherichia coli K-12.

作者信息

McFall E

出版信息

J Bacteriol. 1967 Dec;94(6):1982-8. doi: 10.1128/jb.94.6.1982-1988.1967.

DOI:10.1128/jb.94.6.1982-1988.1967
PMID:4864409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC276930/
Abstract

An episome, F32, which carries the genetic markers dsdA(+), the presumed structural gene for d-serine deaminase, dsdC(+), a regulatory locus governing the synthesis of d-serine deaminase, aroC(+), and purC(+) was obtained from strain AB311 of Escherichia coli K-12, and was used to construct appropriate merodiploids with dsdC markers. In all dsdC / dsdC(+) diploids examined, dsdC was found to be cis dominant, trans recessive, to dsdC(+). In two cases, however, the cis dominance was only partial. Moreover, complementation was observed between one of the dsdC markers which is fully cis dominant and one which is partially cis dominant. Because of the size of the dsdC region, the phenotypes of the mutants, and the partial trans dominance of dsdC(+) over some of the dsdC mutations, it is suggested that the dsdC region specifies a product, but that this product does not move with facility through the cytoplasm

摘要

从大肠杆菌K - 12菌株AB311中获得了一种附加体F32,它携带遗传标记dsdA(+)(推测为d - 丝氨酸脱氨酶的结构基因)、dsdC(+)(一个控制d - 丝氨酸脱氨酶合成的调控位点)、aroC(+)和purC(+),并用于构建带有dsdC标记的合适部分二倍体。在所有检测的dsdC / dsdC(+)二倍体中,发现dsdC对dsdC(+)是顺式显性、反式隐性的。然而,在两个案例中,顺式显性只是部分的。此外,在一个完全顺式显性的dsdC标记和一个部分顺式显性的dsdC标记之间观察到了互补作用。由于dsdC区域的大小、突变体的表型以及dsdC(+)对一些dsdC突变的部分反式显性,表明dsdC区域指定了一种产物,但这种产物在细胞质中不容易移动。

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