Previte J J
J Bacteriol. 1968 Jun;95(6):2165-70. doi: 10.1128/jb.95.6.2165-2170.1968.
The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-, or alcohol-killed preparations. An amount of 5 Mrad is about a 50% end point in terms of inactivation of the lethal lipopolysaccharide or cell-associated determinants. The fever response to radiation-killed salmonellae decreased between 1- and 20-Mrad exposure. The immunogenicity of 1-Mrad-treated cells usually exceeded that of nonirradiated preparations in mouse-protection tests. With increasing radiation doses, there was a dramatic decrease in, but not an abolition of, immunogenicity. Preparations exposed to 20 Mrad which were nonlethal afforded significant protection. The results are interpreted as a reflection of a dissociation of the primary and secondary toxic determinants of endotoxin after irradiation. The data indicate the potential value of radiation sterilization as a means of production of Salmonella vaccine.
研究了高剂量辐射（1、5或20兆拉德）对鼠伤寒沙门氏菌细胞及内毒素的毒性、致热原性和免疫原性的影响。暴露于1、5或20兆拉德辐射后，毒性逐渐降低。1兆拉德辐射处理后的细胞的致死效应大于热灭活、丙酮灭活或酒精灭活制剂。就致死性脂多糖或细胞相关决定簇的失活而言，5兆拉德的剂量约为50%的终点。辐射灭活的沙门氏菌引起的发热反应在1至20兆拉德的暴露剂量之间降低。在小鼠保护试验中，1兆拉德处理的细胞的免疫原性通常超过未辐照制剂。随着辐射剂量的增加，免疫原性显著降低，但并未消除。暴露于20兆拉德且未致死的制剂提供了显著的保护作用。这些结果被解释为辐射后内毒素一级和二级毒性决定簇解离的反映。数据表明辐射灭菌作为生产沙门氏菌疫苗手段的潜在价值。
