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间接荧光抗体技术在评估抗淋巴细胞血清和抗淋巴细胞球蛋白活性中的应用。

The use of the indirect fluorescent antibody technique in assessing the activity of antilymphocytic sera and antilymphocytic globulins.

作者信息

Thomas D, Mosedale B, Zola H

出版信息

Clin Exp Immunol. 1971 Jun;8(6):987-91.

Abstract

Human and mouse lymphocytes have been treated with the appropriate antilymphocytic serum or globulin and the indirect fluorescent technique applied. A method of scoring the degree of fluorescence observed has been devised. A high degree of correlation has been found between the observed fluorescence and the immunosuppressive properties of the various sera and globulin fractions tested.

摘要

已用适当的抗淋巴细胞血清或球蛋白处理人及小鼠淋巴细胞,并应用间接荧光技术。已设计出一种对观察到的荧光程度进行评分的方法。已发现观察到的荧光与所测试的各种血清及球蛋白组分的免疫抑制特性之间存在高度相关性。

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Preparation of antilymphocytic globulin for human use.供人使用的抗淋巴细胞球蛋白的制备。
Transplantation. 1972 Oct;14(4):462-7. doi: 10.1097/00007890-197210000-00009.

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Testing of antihuman lymphocyte sera in chimpanzees and lower primates.
Transplantation. 1969 Sep;8(3):281-90. doi: 10.1097/00007890-196909000-00009.
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Immunosuppressive effects of heterologous antilymphocyte serum in monkeys.
Lancet. 1970 Jan 24;1(7639):167-70. doi: 10.1016/s0140-6736(70)90407-1.

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