Wendt L, Mobach H W
J Bacteriol. 1969 Feb;97(2):640-6. doi: 10.1128/jb.97.2.640-646.1969.
The addition of phenethyl alcohol (PEA) to cultures of male strains of Escherichia coli rapidly prevents the adsorption of the male-specific bacteriophages f1 and f2 to the donor cells. The adsorption of f2 to F pili in cell-free preparations is unaffected by PEA. In a mating system, PEA alters the kinetics of gene transfer in minimal medium but not in broth. Sodium cyanide, azide, and iodoacetate also apparently inhibit f2 adsorption to cells but not to detached F pili. The phage adsorption inhibitory action of PEA is completely reversible in the presence of 100 mug of chloramphenicol per ml.
在雄性大肠杆菌菌株的培养物中添加苯乙醇(PEA)能迅速阻止雄性特异性噬菌体f1和f2吸附到供体细胞上。在无细胞制剂中,f2对F菌毛的吸附不受PEA影响。在交配系统中,PEA改变了在基本培养基中基因转移的动力学,但在肉汤中则没有。氰化钠、叠氮化物和碘乙酸盐显然也能抑制f2吸附到细胞上,但不能抑制其吸附到分离的F菌毛上。在每毫升含有100微克氯霉素的情况下,PEA的噬菌体吸附抑制作用是完全可逆的。