Walker E M, Pittard J
J Bacteriol. 1969 Oct;100(1):319-28. doi: 10.1128/jb.100.1.319-328.1969.
A mutant strain of Escherichia coli K-12 which is unable to transfer genetic material at 42 C has been isolated. Although transfer is inhibited at this temperature, the formation of specific pairs is unaffected. This strain and its derivatives also show an altered sensitivity to certain ribonucleic acid-containing male specific phages at 42 C. Both phenotypic changes are reversed when a derepressed fi(+) R factor is present in the same cell and a revertant which has regained both activities has been obtained. The nature of the temperature-sensitive defect and its effect on the conjugation process are discussed.
已分离出一种大肠杆菌K - 12突变株，该突变株在42℃时无法转移遗传物质。尽管在此温度下转移受到抑制，但特异性配对的形成不受影响。该菌株及其衍生物在42℃时对某些含核糖核酸的雄性特异性噬菌体也表现出敏感性改变。当同一个细胞中存在去阻遏的fi(+) R因子时，这两种表型变化都会逆转，并且已经获得了恢复这两种活性的回复突变体。文中讨论了温度敏感缺陷的性质及其对结合过程的影响。
