两种抗原性不同的人干扰素。
Two antigenically distinct species of human interferon.
作者信息
Havell E A, Berman B, Ogburn C A, Berg K, Paucker K, Vilcek J
出版信息
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2185-7. doi: 10.1073/pnas.72.6.2185.
Rabbit antisera prepared against interferon produced in human fibroblast cell cultures stimulated with poly(1).poly(C) neutralized the activity of interferon preparations produced in various human fibroblast cultures timulated either with poly(1)poly)C) or with viruses. However, these antisera showed no detectable neutralizing activity against interferon produced in cultures of human leukocytes. On the other hand, most rabbit antisera against the human leukocyte interferon were active in neutralizing both homologous interferon and fibroblast interferons. A preparation of antiserum against leukocyte interferon, active against both leukocyte and fibroblast interferons, was shown by affinity chromatography to have two distinct antibody populations, one of which was specific for the fibroblast interferon. We conclude that the heterologous neutralizing activity of sera from rabbits immunized with leukocyte interferon is liekly to be due to the presence of two antigenic species of interferon. The major antigenic species of leukocyte interferon preparations (designated "Le") is distinct from huamn fibroblast interferon. The minor species of leukocyte interferon ("F") is either identical with, or closely related to, interferon produced in human fibroblast cultures.
用聚肌苷酸-聚胞苷酸（poly(1).poly(C)）刺激人成纤维细胞培养物产生的干扰素制备的兔抗血清，能中和在各种人成纤维细胞培养物中用聚肌苷酸-聚胞苷酸（poly(1)poly(C)）或病毒刺激产生的干扰素制剂的活性。然而，这些抗血清对人白细胞培养物中产生 的干扰素没有可检测到的中和活性。另一方面，大多数针对人白细胞干扰素的兔抗血清在中和同源干扰素和成纤维细胞干扰素方面都有活性。通过亲和层析显示，一种针对白细胞干扰素的抗血清制剂，对白细胞和成纤维细胞干扰素都有活性，它有两个不同的抗体群体，其中一个对成纤维细胞干扰素具有特异性。我们得出结论，用白细胞干扰素免疫的兔血清的异源中和活性可能是由于存在两种抗原性的干扰素。白细胞干扰素制剂的主要抗原种类（称为“Le”）与人成纤维细胞干扰素不同。白细胞干扰素的次要种类（“F”）与在人成纤维细胞培养物中产生的干扰素相同或密切相关。
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