嘧啶受限的大肠杆菌的嘧啶库与大分子组成
Pyrimidine pools and macromolecular composition of pyrimidine-limited Escherichia coli.
作者信息
Dennis P P, Herman R K
出版信息
J Bacteriol. 1970 Apr;102(1):118-23. doi: 10.1128/jb.102.1.118-123.1970.
Abstract
The growth rate of a pyrimidine-requiring strain was controlled by limiting the concentration of exogenous orotic acid. As the steady state, pyrimidine-limited growth rate was decreased, the intracellular pyrimidine pools and the total nucleic acid per unit mass of culture also decreased. The ratio of deoxyribonucleic acid to protein remained constant, whereas the ratio of ribonucleic acid to protein decreased 30% over a threefold variation in growth rate (50- to 150-min doubling times). The intracellular uridine triphosphate and cytosine triphosphate pools also decreased (although not coordinately), and the pyrimidine biosynthetic enzymes were derepressed. Cell size was unaffected by pyrimidine-mediated variation of the growth rate.
摘要
通过限制外源性乳清酸的浓度来控制嘧啶需求菌株的生长速率。在稳定状态下，嘧啶限制的生长速率降低，细胞内嘧啶库以及每单位培养物质量的总核酸也减少。脱氧核糖核酸与蛋白质的比例保持恒定，而核糖核酸与蛋白质的比例在生长速率三倍变化（倍增时间为50至150分钟）的过程中下降了30%。细胞内三磷酸尿苷和三磷酸胞苷库也减少（尽管不是协同减少），并且嘧啶生物合成酶被去阻遏。细胞大小不受嘧啶介导的生长速率变化的影响。
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