链霉素对在大肠杆菌核糖体上形成的起始复合物的分解作用。
Breakdown by streptomycin of initiation complexes formed on ribosomes of Escherichia coli.
作者信息
Modolell J, Davis B D
出版信息
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1148-55. doi: 10.1073/pnas.67.3.1148.
Abstract
Streptomycin induces breakdown of the completed 70S initiation complex on ribosomes of Escherichia coli, but it does not interfere with any step in the formation of the complex. Moreover, it does not appear to interact with the ribosome in any special way during initiation, since the kinetics of breakdown are the same whether streptomycin is added before formation of the initiation complex, or after its completion, or (as previously observed) after formation of a polypeptide. fMet-tRNA is released as such, without chain elongation; it is released from a puromycin-reactive ("P") site. Streptomycin thus appears to distort not only the A site of the ribosome (as suggested earlier) but also the P site.
摘要
链霉素可诱导大肠杆菌核糖体上已完成的70S起始复合物分解，但它不干扰该复合物形成过程中的任何步骤。此外，在起始过程中，链霉素似乎并未以任何特殊方式与核糖体相互作用，因为无论在起始复合物形成之前、形成之后添加链霉素，还是（如先前观察到的）在多肽形成之后添加链霉素，分解动力学都是相同的。甲酰甲硫氨酰 - tRNA原样释放，没有链的延伸；它从一个对嘌呤霉素有反应的（“P”）位点释放。因此，链霉素似乎不仅会扭曲核糖体的A位点（如先前所述），还会扭曲P位点。
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本文引用的文献
1
Role of guanosine 5'-triphosphate in the initiation of Peptide synthesis, I. Synthesis of formylmethionyl-puromycin.鸟苷 5'-三磷酸在肽合成起始中的作用，I. 甲酰甲硫氨酰-嘌呤霉素的合成
Proc Natl Acad Sci U S A. 1967 Mar;57(3):759-66. doi: 10.1073/pnas.57.3.759.
2
RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.
3
Purification and properties of initiation factor f-1.
Nature. 1969 Jun 7;222(5197):944-7. doi: 10.1038/222944a0.
4
Junction of the 50S ribosomal subunit with the 30S initiation complex.
Nature. 1968 Oct 19;220(5164):244-7. doi: 10.1038/220244a0.
5
Mechanism of inhibition of ribosomes by streptomycin.链霉素抑制核糖体的机制。
Nature. 1969 Oct 25;224(5217):345-8. doi: 10.1038/224345a0.
6
The ribosome dissociation factor and the ribosome-polysome cycle.核糖体解离因子与核糖体-多聚核糖体循环
Cold Spring Harb Symp Quant Biol. 1969;34:223-30. doi: 10.1101/sqb.1969.034.01.028.
7
Studies of ribosomal subunit exchange.核糖体亚基交换的研究。
Cold Spring Harb Symp Quant Biol. 1969;34:209-20. doi: 10.1101/sqb.1969.034.01.027.
8
The contrasting role of strA and ram gene products in ribosomal functioning.strA和ram基因产物在核糖体功能中的对比作用。
Cold Spring Harb Symp Quant Biol. 1969;34:101-9. doi: 10.1101/sqb.1969.034.01.016.
9
Translation and translocation of defined RNA messengers.
J Mol Biol. 1969 Feb 14;39(3):441-60. doi: 10.1016/0022-2836(69)90137-5.
10
Rapid inhibition of polypeptide chain extension by streptomycin.链霉素对多肽链延伸的快速抑制作用。
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