铁转运化合物肠螯合素的生物合成：无法合成2,3-二羟基苯甲酸的大肠杆菌突变体。
Biosynthesis of the iron-transport compound enterochelin: mutants of Escherichia coli unable to synthesize 2,3-dihydroxybenzoate.
作者信息
Young I G, Langman L, Luke R K, Gibson F
出版信息
J Bacteriol. 1971 Apr;106(1):51-7. doi: 10.1128/jb.106.1.51-57.1971.
Abstract
Mutants of Escherichia coli K-12 blocked in each of the three enzymatic reactions between chorismate and 2,3-dihydroxybenzoate, in the pathway leading to the iron-sequestering compound enterochelin, have been isolated and biochemically characterized. The three genes concerned (designated entA, entB and entC) have been shown to be clustered on the chromosome between purE and gal and to be located near minute 14 by cotransduction with the purE, lip, and fep genes. entA, entB, and entC were shown to be the structural genes for 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, 2,3-dihydro-2,3-dihydroxybenzoate synthetase, and isochorismate synthetase, respectively.
摘要
已分离出大肠杆菌K-12的突变体，这些突变体在分支酸与2,3-二羟基苯甲酸之间的三种酶促反应中均被阻断，这三种酶促反应位于通向铁螯合化合物肠螯合素的途径中，并对其进行了生化特性分析。已证明相关的三个基因（命名为entA、entB和entC）在染色体上位于purE和gal之间成簇排列，并通过与purE、lip和fep基因的共转导定位在约14分钟处。结果表明，entA、entB和entC分别是2,3-二氢-2,3-二羟基苯甲酸脱氢酶、2,3-二氢-2,3-二羟基苯甲酸合成酶和异分支酸合成酶的结构基因。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
[The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].[大肠杆菌中β-半乳糖苷酶（乳糖酶）的生物合成；诱导的特异性]
Biochim Biophys Acta. 1951 Nov;7(4):585-99. doi: 10.1016/0006-3002(51)90072-8.
3
EFFECT OF INTEGRATED SEX FACTOR ON TRANSDUCTION OF CHROMOSOMAL GENES IN ESCHERICHIA COLI.整合性因子对大肠杆菌染色体基因转导的影响
J Bacteriol. 1965 Mar;89(3):680-6. doi: 10.1128/jb.89.3.680-686.1965.
4
Preliminary studies on the isolation and metabolism of an intermediate in aromatic biosynthesis: chorismic acid.芳香族生物合成中间体：分支酸的分离与代谢的初步研究
Biochem J. 1964 Feb;90(2):248-56. doi: 10.1042/bj0900248.
5
The isolation, identification and properties of isochorismic acid. An intermediate in the biosynthesis of 2,3-dihydroxybenzoic acid.异分支酸的分离、鉴定及性质。2,3 - 二羟基苯甲酸生物合成中的一种中间体。
Biochim Biophys Acta. 1969 May 6;177(3):389-400. doi: 10.1016/0304-4165(69)90301-8.
6
The isolation, identification and properties of 2,3-dihydro-2,3-dihydroxybenzoic acid. An intermediate in the biosynthesis of 2,3-dihydroxybenzoic acid.2,3-二氢-2,3-二羟基苯甲酸的分离、鉴定及性质。2,3-二羟基苯甲酸生物合成中的一种中间体。
Biochim Biophys Acta. 1969 May 6;177(3):381-8. doi: 10.1016/0304-4165(69)90300-6.
7
The enzymic and chemical formation of 3,4-dihydro-3,4-dihydroxybenzoic acid: a new compound derived from chorismic acid.3,4-二氢-3,4-二羟基苯甲酸的酶促和化学合成：一种源自分支酸的新化合物。
Biochim Biophys Acta. 1969 Feb 18;177(1):182-3. doi: 10.1016/0304-4165(69)90088-9.
8
The isolation and identification of 2,3-dihydroxybenzoic acid and 2-N,6-N-di-92,3-dihydroxybenzoyl)-L-lysine formed by iron-deficient Azotobacter vinelandii.由缺铁的棕色固氮菌形成的2,3 - 二羟基苯甲酸和2 - N,6 - N - 二 -（2,3 - 二羟基苯甲酰基）- L - 赖氨酸的分离与鉴定
Biochemistry. 1969 Mar;8(3):757-62. doi: 10.1021/bi00831a002.
9
The stereochemistry of intermediates involved in the biosynthesis of 2,3-dihydroxybenzoic acid.
Biochim Biophys Acta. 1969 Apr 1;177(2):348-50. doi: 10.1016/0304-4165(69)90147-0.
10
Enzymic and nonenzymic transformations of chorismic acid and related cyclohexadienes.
Biochim Biophys Acta. 1969 Oct 7;192(1):62-72. doi: 10.1016/0304-4165(69)90010-5.
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