大肠杆菌K-12中的苯丙氨酸生物合成：对分支酸变位酶P-预苯酸脱水酶去阻遏的突变体
Phenylalanine biosynthesis in Escherichia coli K-12: mutants derepressed for chorismate mutase P-prephenate dehydratase.
作者信息
Im S W, Pittard J
出版信息
J Bacteriol. 1971 Jun;106(3):784-90. doi: 10.1128/jb.106.3.784-790.1971.
Abstract
Mutants were isolated which are derepressed for the synthesis of chorismate mutase P-prephenate dehydratase. No other enzymes involved in the synthesis of phenylalanine are derepressed in these strains. These mutants are able to grow in concentrations of o- and p-fluorophenylalanine that inhibit the growth of AB3259, the strain from which they were derived. They also excrete phenylalanine. Genetic analysis shows that the mutations causing this derepression are closely linked to the structural gene for this enzyme (cotransduction frequency of 95% or more with pheA). The gene in which they occur has been designated pheO since this gene has all of the properties predicted for an operator gene controlling the pheA structural gene. Finally, the pheO mutant alleles have been shown to be dominant in diploids.
摘要
分离出了对分支酸变位酶P-预苯酸脱水酶合成去阻遏的突变体。在这些菌株中，参与苯丙氨酸合成的其他酶没有去阻遏。这些突变体能够在抑制其来源菌株AB3259生长的邻氟苯丙氨酸和对氟苯丙氨酸浓度下生长。它们还分泌苯丙氨酸。遗传分析表明，导致这种去阻遏的突变与该酶的结构基因紧密连锁（与pheA的共转导频率为95%或更高）。发生这些突变的基因被命名为pheO，因为该基因具有控制pheA结构基因的操纵基因所预测的所有特性。最后，已证明pheO突变等位基因在二倍体中是显性的。
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